Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York.
Cornell University College of Veterinary Medicine, Cornell University, Ithaca, New York.
J Orthop Res. 2019 May;37(5):1071-1079. doi: 10.1002/jor.24281. Epub 2019 Mar 28.
The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control. Horses were exercised on a high-speed treadmill, and synovial fluid was collected pre-operatively and at serial timepoints until 75 days post-operatively. Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis. Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively. HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75. Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs. Viscosity was significantly reduced after surgery. While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower. Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.
本研究旨在探讨关节骨折后滑液成分和润滑的时间变化。通过在马的腕中关节造成骨软骨骨折来诱导创伤后骨关节炎（PTOA），而对侧肢体作为假手术对照。马在高速跑步机上运动，在术前和术后连续时间点采集滑液，直到术后 75 天。使用夹心 ELISA 测量润滑素和透明质酸（HA）浓度，并通过凝胶电泳分析 HA 的分子量分布。在所有润滑模式下，在第 0、19、33 和 61 天使用商用流变仪和定制摩擦仪测量滑液粘度和软骨摩擦系数。HA 浓度在手术后显著降低，并且高分子量 HA（> 6.1MDa）在研究结束时（第 75 天）并未恢复到术前值。与假手术肢体相比，手术后润滑素浓度增加更多。手术后粘度显著降低。虽然边界和弹流模式摩擦系数没有变化，但代表这些模式之间转变的转变数较低。尽管在 OA 肢体中更为明显，但在假手术肢体中也观察到 HA、HA 分子量分布、粘度和转变数的类似紊乱，这可能是由于关节镜检查期间滑液冲洗所致。©2019 矫形研究协会。由 Wiley Periodicals, Inc. 出版。J 矫形研究。
