Bonnevie Edward D, Galesso Devis, Secchieri Cynthia, Bonassar Lawrence J
Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY.
R&D Department, Fidia Farmaceutici SpA, Padua, Italy.
J Orthop Res. 2018 May;36(5):1456-1464. doi: 10.1002/jor.23782. Epub 2017 Dec 5.
Hyaluronic acid (HA) is widely injected as a viscosupplement in the treatment of osteoarthritis. Despite its extensive use, it is not currently known if cartilage degradation alters how HA-based solutions lubricate the articular surface. In this study we utilized a model of cartilage degradation by IL-1β along with a recently developed framework to study role of cartilage degradation on lubrication by clinically-approved HA-based lubricants with high viscosities. Cartilage explants were cultured up to 8 days with 10 ng/ml IL-1β. After culture, samples were examined histologically, immunohistochemically, biochemically, mechanically, topographically, and tribologically. The tribological testing analyzed both boundary and mixed lubrication modes to assess individual effects of viscosity and boundary lubricating ability. Friction testing was carried out using PBS and two clinically approved HA-based viscosupplements in a cartilage-glass configuration. After culture with IL-1β, boundary mode friction was elevated after both 4 and 8 days. Additionally, friction in mixed mode lubrication, where HA is most effective as a lubricant, was significantly elevated after 8 days of culture. As cartilage became rougher, softer, and more permeable after culture, the boundary mode plateau was extended, and as a result, significantly increased lubricant viscosities or sliding speeds were necessary to achieve effective mixed lubrication. Overall, this study revealed that lubrication of cartilage by HA is degradation-dependent and coincides with changes in mechanics and roughness. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1456-1464, 2018.
透明质酸（HA）作为一种粘弹性补充剂被广泛注射用于骨关节炎的治疗。尽管其应用广泛，但目前尚不清楚软骨降解是否会改变基于HA的溶液对关节表面的润滑方式。在本研究中，我们利用白细胞介素-1β诱导的软骨降解模型以及最近开发的框架，来研究软骨降解对临床认可的高粘度HA基润滑剂润滑作用的影响。将软骨外植体与10 ng/ml白细胞介素-1β一起培养长达8天。培养后，对样本进行组织学、免疫组织化学、生物化学、力学、形貌学和摩擦学检查。摩擦学测试分析了边界润滑和混合润滑模式，以评估粘度和边界润滑能力的个体效应。使用磷酸盐缓冲液（PBS）和两种临床认可的HA基粘弹性补充剂，在软骨-玻璃配置下进行摩擦测试。用白细胞介素-1β培养后，4天和8天后边界模式摩擦均升高。此外，在混合模式润滑中（HA作为润滑剂最有效），培养8天后摩擦显著升高。培养后软骨变得更粗糙、更柔软且渗透性更强，边界模式平台延长，因此，需要显著提高润滑剂粘度或滑动速度才能实现有效的混合润滑。总体而言，本研究表明HA对软骨的润滑作用取决于软骨降解情况，并且与力学和粗糙度的变化一致。© 2017年骨科研究协会。由威利期刊公司出版。《矫形外科学研究》36:1456 - 1464，2018年。
