Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada.
J Orthop Res. 2013 Oct;31(10):1549-54. doi: 10.1002/jor.22399. Epub 2013 May 30.
The objective of this study was to determine changes in (1) proteoglycan 4 (PRG4) and hyaluronan (HA) concentration, (2) HA molecular weight (MW) distribution, and (3) cartilage boundary lubricating ability of synovial fluid (SF) from surgical sham (SHAM), anterior cruciate ligament (ACL)/medial collateral ligament (MCL) transection, and lateral meniscectomy (MEN) in a post-knee surgery ovine model. Ovine SF (oSF) was collected at euthanization 20 weeks after surgery, with the contralateral joint serving as the non-operative control. PRG4 and HA concentration in oSF was measured by sandwich enzyme-linked immunosorbent assay, and HA MW distribution by agarose gel electrophoresis. Cartilage boundary lubricating ability of oSF was measured by a cartilage-cartilage friction test. PRG4 and HA concentration in SHAM, ACL/MCL, and MEN oSF were similar in comparison to the contralateral control (CTRL) oSF. The HA MW distribution in the operated oSF for all ranges were similar to the respective CTRL oSF. The kinetic coefficients of friction in operated and CTRL oSF were similar in all groups, and were significantly lower than saline. These results indicate oSF lubricant composition and function at 20 weeks post-knee surgery were similar to contralateral CTRL, and suggest earlier time points post surgery warrant further investigation.
本研究旨在确定（1）蛋白聚糖 4 (PRG4) 和透明质酸 (HA) 浓度、（2）HA 分子量 (MW) 分布以及（3）滑膜液 (SF) 的软骨边界润滑能力在手术假手术 (SHAM)、前交叉韧带 (ACL)/内侧副韧带 (MCL) 切断术和外侧半月板切除术 (MEN) 后的羊膝关节手术后模型中的变化。在手术后 20 周安乐死时收集羊 SF (oSF)，对侧关节作为非手术对照。通过夹心酶联免疫吸附试验测量 oSF 中的 PRG4 和 HA 浓度，并通过琼脂糖凝胶电泳测量 HA MW 分布。通过软骨-软骨摩擦试验测量 oSF 的软骨边界润滑能力。与对侧对照 (CTRL) oSF 相比，SHAM、ACL/MCL 和 MEN oSF 中的 PRG4 和 HA 浓度相似。在所有范围内，手术 oSF 的 HA MW 分布与各自的 CTRL oSF 相似。在所有组中，手术和 CTRL oSF 的动摩擦系数相似，均明显低于生理盐水。这些结果表明，膝关节手术后 20 周时 oSF 润滑剂的组成和功能与对侧 CTRL 相似，这表明更早的术后时间点值得进一步研究。
