Walser Marion, Oscarsson Jan, Åberg Maria A I, Svensson Johan, Isgaard Jörgen, Åberg N David
Department of Internal Medicine, Institute of Medicine, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
AstraZeneca Gothenburg, Mölndal, Sweden.
Neuro Endocrinol Lett. 2019 Feb;39(7):525-531.
Growth hormone (GH) increases insulin-like growth factor I (IGF-I) production and both hormones affect hippocampal plasticity. We have previously shown that Hbb and Alas2 in the rat hippocampus were robustly regulated by GH-infusions for six days, whereas other transcripts were weakly affected. Here, we explored the effects of prolonged GH administration on transcripts linked to neuroprotection and investigated whether serum IGF-I administration may exert similar effects.
Hypophysectomised female rats were infused with GH or IGF-I for 19 days. Hbb, Alas2 and seven additional GH- and IGF-I-related transcripts were quantified by Q-RT-PCR in rat hippocampus.
Three transcripts, Hbb, Alas2, and Alox15 were increased by both GH and IGF-I administration. The other transcripts were marginally affected.
The 19-day GH-infusion induced similar effects as those reported after 6-day GH treatment, with the addition of the regulation of transcript Alox15. IGF-I induced altered gene expression in relation to its effect on weight gain. This study underlines that there is an entity of transcripts involved in neuroprotection and vascular tone that is regulated by both systemic GH and IGF-I. For other transcripts, the longer duration of this study did not significantly enhance the marginal effects of GH administration seen previously.
生长激素（GH）可增加胰岛素样生长因子I（IGF-I）的产生，且这两种激素均影响海马可塑性。我们之前已经表明，大鼠海马中的Hbb和Alas2在连续6天输注GH后受到强烈调节，而其他转录本受到的影响较弱。在此，我们探讨了长期给予GH对与神经保护相关转录本的影响，并研究了给予血清IGF-I是否可能产生类似的效果。
对去垂体的雌性大鼠输注GH或IGF-I，持续19天。通过Q-RT-PCR对大鼠海马中的Hbb、Alas2以及另外7种与GH和IGF-I相关的转录本进行定量分析。
给予GH和IGF-I均使三种转录本Hbb、Alas2和Alox15增加。其他转录本受到的影响较小。
19天的GH输注诱导的效果与6天GH治疗后报道的效果相似，此外还对转录本Alox15进行了调节。IGF-I诱导的基因表达改变与其对体重增加的影响有关。本研究强调，存在一组与神经保护和血管张力相关的转录本，它们受到全身GH和IGF-I的调节。对于其他转录本，本研究较长的持续时间并未显著增强先前观察到的GH给药的微小效果。
