Department of Gastroenterology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, 214023 Jiangsu Province, China.
Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, 214023 Jiangsu Province, China.
Biomed Res Int. 2020 Dec 1;2020:5481653. doi: 10.1155/2020/5481653. eCollection 2020.
Acute radiation enteritis (ARE) is a common complication with radiotherapy for pelvic and abdominal malignancy. This research is designed to investigate the efficacy of Tong-Xie-Yao-Fang (TXYF) on ARE and to explore the underlying mechanisms by microarray analysis. The ARE rat model was established by a single abdominal irradiation with a gamma-ray dose of 10 Gy. Next, the ARE rats were treated with distilled water, TXYF, and glutamine by gavage for 7 consecutive days according to the scheduled groups. For each group, the jejunal tissue was taken at 6 h after gastric lavage. The morphology of intestinal tissue was observed by hematoxylin and eosin (H&E) stain under a light microscope. The height of the villus and the thickness of the whole layer of the TXYF-treated groups were significantly ameliorative than that of the model control group. The transcriptome analysis was produced using the Agilent SurePrint G3 Rat GE V2.0 microarray. A total of 90 differentially expressed genes (DEGs), including 48 upregulated genes and 42 downregulated genes, were identified by microarray and bioinformatics analysis. Protein-protein interaction (PPI), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) were conducted to explore the possible mechanisms of DEGs taking part in the TXYF-mediated therapeutic process for ARE. In conclusion, we reveal that TXYF has a protective effect on the intestinal tissue of rats with ARE and summarize several DEGs, suggesting the possible mechanisms of TXYF-mediated efficacy for ARE.
急性放射性肠炎（ARE）是盆腔和腹部恶性肿瘤放射治疗的常见并发症。本研究旨在通过基因芯片分析探讨通泄遥方（TXYF）治疗 ARE 的疗效及其作用机制。采用单次 10Gyγ射线腹部照射建立 ARE 大鼠模型，然后灌胃给予蒸馏水、TXYF 和谷氨酰胺，连续 7 天，按预定分组进行治疗。每组大鼠于灌胃后 6h 取空肠组织，采用苏木精-伊红（H&E）染色观察肠组织形态学变化。TXYF 治疗组的绒毛高度和全层厚度明显优于模型对照组。采用 Agilent SurePrint G3 Rat GE V2.0 基因芯片进行转录组分析。通过微阵列和生物信息学分析，共鉴定出 90 个差异表达基因（DEGs），包括 48 个上调基因和 42 个下调基因。通过蛋白-蛋白相互作用（PPI）、基因本体（GO）和京都基因与基因组百科全书（KEGG）分析，探讨 DEGs 参与 TXYF 介导的 ARE 治疗过程的可能机制。总之，本研究揭示了 TXYF 对 ARE 大鼠肠组织具有保护作用，并总结了几个 DEGs，提示了 TXYF 介导 ARE 疗效的可能机制。
