Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India.
FEBS Lett. 2019 Apr;593(7):703-718. doi: 10.1002/1873-3468.13356. Epub 2019 Mar 22.
Eukaryotes employ a subset of dynamins to mediate mitochondrial fusion and fission dynamics. Here we report the molecular evolution and diversification of the dynamin-related mitochondrial proteins that drive the fission (Drp1) and the fusion processes (mitofusin and OPA1). We demonstrate that the three paralogs emerged concurrently in an early mitochondriate eukaryotic ancestor. Furthermore, multiple independent duplication events from an ancestral bifunctional fission protein gave rise to specialized fission proteins. The evolutionary history of these proteins is marked by transformations that include independent gain and loss events occurring at the levels of entire genes, specific functional domains, and intronic regions. The domain level variations primarily comprise loss-gain of lineage specific domains that are present in the terminal regions of the sequences.
真核生物利用一组动力蛋白来介导线粒体融合和裂变动力学。在这里，我们报告了驱动裂变（Drp1）和融合过程（线粒体融合蛋白和 OPA1）的动力蛋白相关线粒体蛋白的分子进化和多样化。我们证明，这三个直系同源物在早期的线粒体真核生物祖先中同时出现。此外，从祖先的双功能裂变蛋白中发生的多个独立重复事件产生了专门的裂变蛋白。这些蛋白质的进化历史以转化为特征，包括整个基因、特定功能域和内含子区域的独立获得和丢失事件。在结构域水平上的变化主要包括谱系特异性结构域的获得和丢失，这些结构域存在于序列的末端区域。
