Lucas M, Solano F
Int J Biochem. 1986;18(6):525-9. doi: 10.1016/0020-711x(86)90163-1.
Subcellular compartmentation of calcium has been studied in digitonin-treated pigeon erythrocytes. The following calcium pools could be detected: A non-vesicular and tightly bound pool of calcium able to reach values equivalent to 5 mumol calcium/ml cells that required millimolar calcium concentrations. A vesicular calcium pool with high calcium affinity that had properties similar to mitochondrial calcium transport. Extracellularly added ATP was strongly hydrolyzed by intact pigeon erythrocytes in the presence of either magnesium or calcium. The hydrolysis of ATP was not coupled to fluxes of either 45Ca2+ or 86Rb+ and most probably took place inside the cells.
已在洋地黄皂苷处理的鸽红细胞中研究了钙的亚细胞区室化。可检测到以下钙池：一个非囊泡且紧密结合的钙池，其钙含量可达相当于5微摩尔钙/毫升细胞的值，这需要毫摩尔级的钙浓度。一个具有高钙亲和力的囊泡钙池，其性质类似于线粒体钙转运。在镁或钙存在的情况下，完整的鸽红细胞会强烈水解细胞外添加的ATP。ATP的水解与45Ca2+或86Rb+的通量均未偶联，很可能发生在细胞内部。
