Zhao J Y, Zhao X, Yang Y, Zhang Y, Zhao S
Department of Thoracic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Department of Biotherapy Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Zhong Liu Za Zhi. 2019 Feb 23;41(2):97-101. doi: 10.3760/cma.j.issn.0253-3766.2019.02.004.
To investigate the effects of human umbilical cord mesenchymal stem cells (MSCs) on the proliferation, apoptosis, migration, invasion and stemness of esophageal cancer EC1 cells. Human umbilical cord mesenchymal stem cells were isolated and cultured and cell phenotype was identified by flow cytometry. MSCs or their conditioned medium were co-cultured with esophageal cancer EC1 cells. The effects on the proliferation, apoptosis, migration, invasion and stemness of EC1 cells were examined by cell counting kit-8 (CCK-8), flow cytometry, Transwell, quantitative real-time polymerase chain reaction (RT-qPCR) and spheroid formation assays. MSCs inhibited the proliferation of EC1 cells in a concentration dependent manner. When the ratio of MSCs to EC1 cells was 0∶1, 1∶1, 2∶1, 5∶1, the apoptotic rates of EC1 cells were (4.07±0.34)%, (8.90±0.36)%, (10.80±0.50)% and (15.23±1.06)%, respectively, suggesting that MSCs promoted the apoptosis of EC1 cells in a concentration dependent manner (all <0.05). The expression levels of OCT2, SOX2, KLF4, CXCR4 and CXCR7 in EC1 cells cultured in 80% conditioned medium were 0.53±0.03, 0.49±0.02, 0.73±0.09, 0.57±0.05 and 0.24±0.02, respectively, which were lower than those in the regular medium group (all <0.05). The numbers of migrated cells in regular medium as well as 10%, 40%, and 80% conditioned medium were 287.3±21.6, 280.7±15.5, 264.3±16.8, and 257.7±8.0, respectively. Meanwhile, the numbers of invasive cells were 194.3±16.6, 213.7±24.3, 221.0±16.0, (252.0±20.4), respectively. There was no significant difference between the groups (all >0.05). Human umbilical cord mesenchymal stem cells can inhibit the proliferation, promote apoptosis and reduce the stemness, and have no significant effect on the migration and invasion of EC1 cells.
探讨人脐带间充质干细胞（MSCs）对食管癌EC1细胞增殖、凋亡、迁移、侵袭及干性的影响。分离培养人脐带间充质干细胞，采用流式细胞术鉴定细胞表型。将MSCs或其条件培养基与食管癌EC1细胞共培养。采用细胞计数试剂盒-8（CCK-8）、流式细胞术、Transwell、定量实时聚合酶链反应（RT-qPCR）和球体形成试验检测对EC1细胞增殖、凋亡、迁移、侵袭及干性的影响。MSCs以浓度依赖性方式抑制EC1细胞的增殖。当MSCs与EC1细胞的比例为0∶1、1∶1、2∶1、5∶1时，EC1细胞的凋亡率分别为（4.07±0.34）%、（8.90±0.36）%、（10.80±0.50）%和（15.23±1.06）%，提示MSCs以浓度依赖性方式促进EC1细胞凋亡（均P<0.05）。在80%条件培养基中培养的EC1细胞中，OCT2、SOX2、KLF4、CXCR4和CXCR7的表达水平分别为0.53±0.03、0.49±0.02、0.73±0.09、0.57±0.05和0.24±0.02，均低于常规培养基组（均P<0.05）。常规培养基以及10%、40%和80%条件培养基中迁移细胞数分别为287.3±21.6、280.7±15.5、264.3±16.8和257.7±8.0。同时，侵袭细胞数分别为194.3±16.6、213.7±24.3、221.0±16.0、（252.0±20.4）。各组间差异均无统计学意义（均P>0.05）。人脐带间充质干细胞可抑制EC1细胞增殖，促进凋亡并降低其干性，对EC1细胞的迁移和侵袭无明显影响。
