Chawla T C, Atreyi M, Jailkhani B L
Int Arch Allergy Appl Immunol. 1986;80(2):221-3. doi: 10.1159/000234055.
The saline extract (SE) of Mycobacterium tuberculosis H37Ra yielded four major fractions on Sephadex G-100, with average molecular weights of 48,000 (fraction I, F I) 32,000 (fraction II, F II), 15,000 (fraction III, F III) and 5,000 (fraction IV, F IV). FI and FII gave single bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, showed complete immunological cross-reactivity with each other and were more potent in reacting with antibodies in patients' (tuberculosis) sera than sonicate, SE, purified protein derivative, F III and F IV when tested in a competition enzyme-linked immunosorbent assay.
结核分枝杆菌H37Ra的盐水提取物（SE）在Sephadex G - 100上产生了四个主要级分，平均分子量分别为48,000（级分I，FI）、32,000（级分II，FII）、15,000（级分III，FIII）和5,000（级分IV，FIV）。FI和FII在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳上呈现单一条带，彼此表现出完全的免疫交叉反应性，并且在竞争酶联免疫吸附试验中进行测试时，与患者（结核病）血清中的抗体反应比超声提取物、SE、纯化蛋白衍生物、FIII和FIV更有效。
