Gandhi B M, Bhargava D K, Irshad M, Chawla T C, Dube A, Tandon B N
Tubercle. 1986 Sep;67(3):219-24. doi: 10.1016/s0041-3879(86)80028-9.
Enzyme linked protein-A has been used to develop an enzyme-linked immunosorbent assay (ELISA) to detect circulating IgG antibodies to Mycobacterium tuberculosis. The specific binding of protein-A to IgG fractions through Fc receptors, makes the test more specific for detection of total IgG antibodies. The ELISA system has been used for detection of circulating antibodies to M. tuberculosis H37Ra in 22 patients with histologically proven intestinal tuberculosis and 88 healthy controls, in addition to 7 diseased controls. The ELISA has been found to be a sensitive test as it was positive in all 22 patients with intestinal tuberculosis. Its specificity was 85% in Indian controls and 97% in Norwegian controls. The test is easy to perform and may be recommended for the serological diagnosis of intestinal tuberculosis.
酶联蛋白A已被用于开发一种酶联免疫吸附测定（ELISA），以检测循环中的结核分枝杆菌IgG抗体。蛋白A通过Fc受体与IgG组分的特异性结合，使得该检测对于总IgG抗体的检测更具特异性。该ELISA系统除用于检测7例疾病对照外，还用于检测22例经组织学证实为肠结核的患者及88例健康对照者体内循环的抗结核分枝杆菌H37Ra抗体。ELISA被发现是一种敏感的检测方法，因为它在所有22例肠结核患者中均呈阳性。其在印度对照中的特异性为85%，在挪威对照中的特异性为97%。该检测易于操作，可推荐用于肠结核的血清学诊断。
