Gilbertsen R B
Immunopharmacology. 1986 Apr;11(2):69-77. doi: 10.1016/0162-3109(86)90026-3.
Concentrations of the acute phase reactant haptoglobin were quantitated in the serum of rats using a commercially available antihuman haptoglobin radial immunodiffusion kit. That this antiserum reacted with rat haptoglobin was shown through the techniques of Ouchterlony immunodiffusion and immunoelectrophoresis. Haptoglobin levels were increased seven days after immunization of rats with type II collagen plus incomplete Freund's adjuvant (ICFA) and peaked on day 14. However, even six weeks post-immunization the concentration of haptoglobin was elevated in arthritic rats. A significant correlation was observed between the concentration of serum haptoglobin and the severity of disease (arthritic index) in rats immunized six weeks previously with type II collagen plus ICFA. The effects of antiinflammatory and antirheumatic therapy on arthritic index and serum haptoglobin concentration were determined using a therapeutic dosing protocol. Under these conditions, the known antiarthritic effects of nonsteroidal antiinflammatory drugs, steroids and immunosuppressive agents in this model were confirmed. Of these agents, only the nonsteroidal drugs reduced serum haptoglobin; hydrocortisone, cyclophosphamide and azathioprine elevated haptoglobin. Aurothioglucose, auranofin, and chloroquine, members of the class of disease-modifying antirheumatic drugs, had a general tendency to exacerbate disease, but had minimal effect on serum haptoglobin. D-Penicillamine had little effect on arthritic index and haptoglobin. These results suggest that, while haptoglobin levels do correlate with the intensity of hindpaw swelling, measurement of haptoglobin may not be an accurate indicator of the underlying disease processes in the collagen arthritis model.
使用市售的抗人触珠蛋白放射免疫扩散试剂盒对大鼠血清中的急性期反应物触珠蛋白浓度进行定量。通过欧氏免疫扩散和免疫电泳技术表明，这种抗血清与大鼠触珠蛋白发生反应。在用II型胶原加不完全弗氏佐剂（ICFA）免疫大鼠7天后，触珠蛋白水平升高，并在第14天达到峰值。然而，即使在免疫后六周，关节炎大鼠的触珠蛋白浓度仍升高。在六周前用II型胶原加ICFA免疫的大鼠中，观察到血清触珠蛋白浓度与疾病严重程度（关节炎指数）之间存在显著相关性。使用治疗给药方案确定抗炎和抗风湿治疗对关节炎指数和血清触珠蛋白浓度的影响。在这些条件下，证实了该模型中已知的非甾体抗炎药、类固醇和免疫抑制剂的抗关节炎作用。在这些药物中，只有非甾体药物降低了血清触珠蛋白；氢化可的松、环磷酰胺和硫唑嘌呤升高了触珠蛋白。金硫葡萄糖、金诺芬和氯喹是改善病情抗风湿药类的成员，它们总体上有加重疾病的趋势，但对血清触珠蛋白影响最小。D-青霉胺对关节炎指数和触珠蛋白影响不大。这些结果表明，虽然触珠蛋白水平确实与后爪肿胀的程度相关，但在胶原性关节炎模型中，触珠蛋白的测量可能不是潜在疾病过程的准确指标。
