Kiessling A A, Hughes W H, Blankevoort M R
J Am Vet Med Assoc. 1986 Apr 15;188(8):829-32.
Eighteen mature, purebred (Saanen, Alpine, Nubian, La Mancha) dairy does were estrus-regulated with progesterone-impregnated vaginal pessaries. On treatment days 16 to 18, superovulation of 6 does was attempted with pregnant mare's serum gonadotropin (PMSG), and of 12 does with follicle stimulating hormone (FSH). Estrus was induced by progesterone withdrawal on days 18 to 20 and the does were mated to bucks of proven fertility. Embryos were recovered from oviduct flushings on the fourth day after mating. The yield of 4- to 12-cell embryos with FSH superovulation was 17(+/- 2), significantly greater (P less than 0.001) than with PMSG, which was 2(+/- 2). Thirty-six embryos from 3 FSH donor does were transferred to 14 recipients that were estrus-regulated to the donor cycles with the same progesterone pessary regimen. Eleven (78%) of the recipients became pregnant and delivered 21 (70%) kids from 40 embryos, a mean of 7(+/- 2)/donor.
18只成年纯种(萨能、阿尔卑斯、努比亚、拉曼查)母羊用含孕酮的阴道栓进行发情调控。在治疗的第16至18天,6只母羊尝试用孕马血清促性腺激素(PMSG)进行超数排卵,12只母羊用促卵泡激素(FSH)进行超数排卵。在第18至20天通过撤去孕酮诱导发情,母羊与经证实有生育能力的公羊交配。在交配后第四天从输卵管冲洗液中回收胚胎。FSH超数排卵获得4至12细胞胚胎的产量为17(±2),显著高于(P<0.001)PMSG组的2(±2)。来自3只FSH供体母羊的36枚胚胎被移植到14只受体母羊,这些受体母羊用相同的孕酮栓方案进行发情调控以同步于供体周期。11只(78%)受体母羊怀孕,40枚胚胎产下21只(70%)羔羊,平均每只供体母羊产7(±2)只。