Obaru K, Fukuda M, Maeda S, Shimada K
J Biochem. 1986 Mar;99(3):885-94. doi: 10.1093/oxfordjournals.jbchem.a135549.
A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar lymphocytes with either TPA or PHA increased the amount of pLD78-specific RNA by about 10-fold, and simultaneous stimulation with TPA and PHA, by at least 30-fold. Analysis of the pLD78 cDNA sequence revealed that it codes for a polypeptide consisting of 92 amino acid residues, including a putative signal sequence. Moreover, the sequence of the 5' flanking region of the nuclear DNA encoding for the pLD78 cDNA showed a significant homology with the corresponding regions of the human interleukin 2 and immune interferon genes.
从由经12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）和植物血凝素（PHA）刺激的人扁桃体淋巴细胞的聚腺苷酸加尾（poly(A)+）RNA构建的cDNA文库中，分离出一个可被肿瘤启动子TPA或T细胞有丝分裂原PHA诱导的cDNA克隆，命名为pLD78。用TPA或PHA刺激扁桃体淋巴细胞，可使pLD78特异性RNA的量增加约10倍，而同时用TPA和PHA刺激，则至少增加30倍。对pLD78 cDNA序列的分析表明，它编码一个由92个氨基酸残基组成的多肽，包括一个推定的信号序列。此外，编码pLD78 cDNA的核DNA的5'侧翼区域序列与人类白细胞介素2和免疫干扰素基因的相应区域显示出显著的同源性。
