Hirano T, Fujimoto K, Teranishi T, Nishino N, Onoue K, Maeda S, Shimada K
J Immunol. 1984 May;132(5):2165-7.
TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone.
单独使用佛波酯（TPA）不会诱导人扁桃体淋巴细胞产生白细胞介素2（IL - 2），但能将植物血凝素（PHA）诱导的IL - 2产生量提高7倍。通过检测非洲爪蟾卵母细胞中可翻译的IL - 2信使核糖核酸（mRNA）的量，以及使用IL - 2互补脱氧核糖核酸（cDNA）作为探针进行Northern印迹分析，证明了TPA的作用是由于IL - 2 mRNA增加。通过这些方法表明，单独使用TPA不会诱导任何显著的IL - 2 mRNA合成，但与PHA一起添加时，与单独由PHA诱导的水平相比，它使IL - 2 mRNA水平至少提高了10倍。
