Xiong Guosheng, Li Liyin, Chen Xiaobo, Song Sinuo, Zhao Yunping, Cai Wenke, Peng Jingping
Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, People's Republic of China,
Department of Hematology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, People's Republic of China.
Onco Targets Ther. 2019 Feb 12;12:1171-1180. doi: 10.2147/OTT.S182242. eCollection 2019.
The purpose of this study is to consider the function of cytoskeleton-associated protein 2-like (CKAP2L) in lung adenocarcinoma (LAD) development and its prognostic value.
The mRNA expression of CKAP2L and its correlation with clinical factors in LAD patients were analyzed from the data taken from The Cancer Genome Atlas and The First Affiliated Hospital of Kunming Medical University. We constructed H460 and A549 cell lines with silenced CKAP2L using RNA interference. Cell counting kit-8 assay and colony formation assays were carried out to determine the function of CKAP2L in H460 and A549 cell proliferation. Transwell and wound healing assays were applied to determine the effect of CKAP2L on H460 and A549 cell invasion and migration. The influences of CKAP2L on mitogen-activated protein kinase signaling pathway-related proteins were tested by Western blotting.
CKAP2L expression is enhanced in LAD tissues and is predictive of poor prognosis in LAD patients. High expression of CKAP2L is associated with stage (<0.001), lymph node status (=0.002), and metastasis (=0.025). Depletion of CKAP2L dramatically suppressed the proliferation, migration, and invasion of H460 and A549 cells. Moreover, the ratio of p-MEK/ MEK and p-ERK/ERK reduced obviously in A549 cells after depleting CKAP2L.
Our findings implied that CKAP2L might be a promoter of LAD and could serve as a predictor for LAD patients.
本研究旨在探讨细胞骨架相关蛋白2样蛋白（CKAP2L）在肺腺癌（LAD）发生发展中的作用及其预后价值。
从癌症基因组图谱以及昆明医科大学第一附属医院获取的数据中，分析LAD患者中CKAP2L的mRNA表达及其与临床因素的相关性。我们使用RNA干扰构建了CKAP2L沉默的H460和A549细胞系。采用细胞计数试剂盒-8法和集落形成试验来确定CKAP2L在H460和A549细胞增殖中的作用。运用Transwell试验和伤口愈合试验来确定CKAP2L对H460和A549细胞侵袭和迁移的影响。通过蛋白质免疫印迹法检测CKAP2L对丝裂原活化蛋白激酶信号通路相关蛋白的影响。
CKAP2L在LAD组织中表达增强，提示LAD患者预后不良。CKAP2L的高表达与分期（<0.001）、淋巴结状态（=0.002）和转移（=0.025）相关。CKAP2L的缺失显著抑制了H460和A549细胞的增殖、迁移和侵袭。此外，在A549细胞中敲低CKAP2L后，p-MEK/MEK和p-ERK/ERK的比值明显降低。
我们的研究结果表明，CKAP2L可能是LAD的一个促进因子，可作为LAD患者的一个预测指标。
