Tretbar Sandy, Krausbeck Peter, Müller Anja, Friedrich Michael, Vaxevanis Christoforos, Bukur Juergen, Jasinski-Bergner Simon, Seliger Barbara
Martin Luther University Halle-Wittenberg, Institute for Medical Immunology, 06112 Halle, Germany.
State Hospital, Healthcare Centre Glantal, 55590 Meisenheim, Germany.
Oncotarget. 2019 Feb 19;10(15):1507-1524. doi: 10.18632/oncotarget.26682.
Epithelial-to-mesenchymal transition (EMT) is a crucial step in cancer progression and the number one reason for poor prognosis and worse overall survival of patients. Although this essential process has been widely studied in many solid tumors as e.g. melanoma and breast cancer, more detailed research in renal cell carcinoma (RCC) is required, especially for the major EMT-inducer transforming growth factor beta (TGF-β). Here, we provide a study of six different RCC cell lines of two different RCC subtypes and their response to recombinant TGF-β1 treatment. We established a model system shifting the cells to a mesenchymal cell type without losing their mesenchymal character even in the absence of the external stimulus. This model system forms a solid basis for future studies of the EMT process in RCCs to better understand the molecular basis of this process responsible for cancer progression.
上皮-间质转化（EMT）是癌症进展中的关键步骤，也是患者预后不良和总体生存率较差的首要原因。尽管这一重要过程已在许多实体瘤（如黑色素瘤和乳腺癌）中得到广泛研究，但在肾细胞癌（RCC）方面仍需要更详细的研究，尤其是针对主要的EMT诱导因子转化生长因子β（TGF-β）。在此，我们对两种不同RCC亚型的六种不同RCC细胞系及其对重组TGF-β1治疗的反应进行了研究。我们建立了一个模型系统，可将细胞转变为间充质细胞类型，即使在没有外部刺激的情况下也不会丧失其间充质特性。该模型系统为未来研究RCC中的EMT过程奠定了坚实基础，以便更好地理解这一导致癌症进展的过程的分子基础。
