Alvarez Carlos A, Cobb Brian A
Case Western Reserve University School of Medicine, Cleveland, OH, USA.
Methods Mol Biol. 2019;1954:25-35. doi: 10.1007/978-1-4939-9154-9_3.
Capsular polysaccharides are a dominant class of antigens from bacteria, both pathogenic and symbiotic or commensal. With the rise of awareness for the influence of the microbiota over immune system development and immune homeostasis, analysis of the antigens is more important than ever. Here we describe a method for the isolation of capsular polysaccharide from gram-negative bacteria, with the purification of polysaccharide from the commensal bacterium Bacteroides fragilis serving as an example. The method efficiently removes all detectable endotoxins and other lipid components, proteins, and nucleic acids, providing a source of capsular polysaccharide for immunologic study.
荚膜多糖是细菌（包括致病菌、共生菌或共栖菌）的主要抗原类型。随着人们对微生物群对免疫系统发育和免疫稳态影响的认识不断提高，对这些抗原的分析比以往任何时候都更加重要。在此，我们描述了一种从革兰氏阴性菌中分离荚膜多糖的方法，并以从共生菌脆弱拟杆菌中纯化多糖为例进行说明。该方法能有效去除所有可检测到的内毒素以及其他脂质成分、蛋白质和核酸，为免疫学研究提供了一种荚膜多糖来源。
