Kurt-Jones E A, Virgin H W, Unanue E R
J Immunol. 1986 Jul 1;137(1):10-4.
We examined the expression of a membrane form of interleukin 1 (IL 1) by macrophages. Murine peritoneal macrophages fixed immediately after harvesting, in suspension, did not show membrane IL 1. Membrane IL 1 was expressed after a 3-hr culture on plastic dishes. These findings allowed us to examine some conditions in vivo that may trigger the expression of this protein; that is, by fixing the macrophages in suspension we could determine if a given stimulus had an effect. We found that membrane IL 1 was expressed briefly after administration of live or dead Listeria monocytogenes or endotoxin. Serum proteins were ineffective. At the time of maximal activation of macrophages by live Listeria, membrane IL 1 was not expressed. Analysis of in vitro conditions indicated that expression of membrane IL 1 and Ia molecules could be dissociated. In culture, recombinant interferon-gamma induced Ia but no membrane IL 1. Uptake of dead Listeria organisms had no effect on Ia but triggered membrane IL 1. The stimulation of membrane IL 1 was not caused by phagocytosis per se: latex particles were ineffective. Opsonized red cells stimulated membrane IL 1 on macrophages that were activated in vivo by inflammatory stimuli.
我们检测了巨噬细胞中白细胞介素1（IL-1）膜形式的表达。收获后立即固定于悬浮状态的小鼠腹腔巨噬细胞未显示膜IL-1。在塑料培养皿上培养3小时后，膜IL-1开始表达。这些发现使我们能够研究体内可能触发该蛋白表达的一些条件；也就是说，通过将巨噬细胞固定于悬浮状态，我们可以确定给定刺激是否有作用。我们发现，在给予活的或死的单核细胞增生李斯特菌或内毒素后，膜IL-1会短暂表达。血清蛋白无效。在活的李斯特菌使巨噬细胞达到最大激活时，膜IL-1未表达。体外条件分析表明，膜IL-1和Ia分子的表达可以分离。在培养中，重组干扰素-γ诱导Ia表达，但不诱导膜IL-1表达。摄取死的李斯特菌对Ia无影响，但触发膜IL-1表达。膜IL-1的刺激不是由吞噬作用本身引起的：乳胶颗粒无效。经调理的红细胞刺激了在体内被炎症刺激激活的巨噬细胞上的膜IL-1。
