巴基斯坦健康人群血液中的 Merkel 细胞多瘤病毒 DNA 序列。
Merkel cell polyomavirus DNA sequences in the blood of healthy population of Pakistan.
机构信息
School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
Institute of Microbiology, University of Agriculture Faisalabad, Pakistan.
出版信息
Future Microbiol. 2019 May;14:599-608. doi: 10.2217/fmb-2018-0314. Epub 2019 Mar 13.
This study aimed at detecting and quantifying Merkel cell polyomavirus (MCPyV) viral loads in the peripheral blood of healthy Pakistani individuals. A total of 221 whole blood samples obtained from healthy individuals were examined by qPCR. MCPyV was detected in the peripheral blood of 31.2% healthy individuals. The rate of MCPyV positivity decreased from young (36%), to middle (33.7%) and elder (25.3%) age groups. Our data revealed higher prevalence of MCPyV in women (43.93%) than men (25.80%). The MCPyV viral load was calculated in the range of 0.06 -11 copies/ng of isolated DNA. The MCPyV viral load increased from young (median = 3.35) to elder (median = 5.66) age groups. The MCPyV circulate at a higher frequency by residing dormant in certain blood cells, which might act as potential vehicles for the spread of MCPyV infection among general population.
本研究旨在检测和定量分析健康巴基斯坦个体外周血中的 Merkel 细胞多瘤病毒（MCPyV）病毒载量。共检测了 221 份来自健康个体的全血样本，采用 qPCR 法进行检测。31.2%的健康个体外周血中检测到 MCPyV。MCPyV 阳性率从年轻（36%）、中年（33.7%）和老年（25.3%）年龄组逐渐降低。我们的数据显示，女性（43.93%）的 MCPyV 阳性率高于男性（25.80%）。MCPyV 病毒载量的计算范围为 0.06-11 拷贝/ng 分离 DNA。MCPyV 病毒载量从年轻（中位数=3.35）到老年（中位数=5.66）年龄组逐渐增加。MCPyV 以休眠状态存在于某些血细胞中，其循环频率更高，这可能成为 MCPyV 感染在普通人群中传播的潜在载体。
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