Division of Molecular Pathology, Department of Pathology, Tottori University Faculty of Medicine, Yonago, Tottori 683-8503, Japan. hayashik @ med.tottori-u.ac.jp
Intervirology. 2013;56(1):1-5. doi: 10.1159/000338620. Epub 2012 Sep 12.
It has recently been shown that approximately 80% of Merkel cell carcinomas harbor a novel polyomavirus named Merkel cell polyomavirus (MCPyV). MCPyV has been detected in human tissue samples. However, detailed distribution of MCPyV in non-neoplastic Japanese human tissues remains unclear. To address this, we used single or real-time quantitative polymerase chain reaction (PCR) for 41 autopsy cases. PCR revealed MCPyV-DNA in non-neoplastic samples: total, 29/41 (71%); adult, 29/39 (74%); fetus or infant, 0/2; men, 24/28 (86%); women, 5/13 (38%); total human tissues, 66/572 (12%); skin, 8/15 (53%); adrenal gland, 9/33 (27%), and other 16 organs (4-25%). This study first reported the presence of MCPyV-DNA in non-neoplastic tissues of thyroid gland, adrenal gland, spleen, bone marrow, stomach, gallbladder, pancreas, heart, and aorta. PCR revealed that viral load ranged from 0.00026 to 0.22 in all MCPyV-positive tissues compared with Merkel cell carcinoma samples. These detailed PCR data showed higher prevalence of MCPyV infection in Japanese men than women (p = 0.004) and broad distribution of MCPyV with low viral load in more non-neoplastic human tissues than in the previous reports. These data provide valuable insights for further studies of MCPyV infection and MCPyV-related diseases.
最近的研究表明，大约 80%的 Merkel 细胞癌携带有一种新型的多瘤病毒，称为 Merkel 细胞多瘤病毒（MCPyV）。已经在人类组织样本中检测到 MCPyV。然而，MCPyV 在非肿瘤性日本人体组织中的详细分布情况尚不清楚。为了解决这个问题，我们使用单重或实时定量聚合酶链反应（PCR）对 41 例尸检病例进行了检测。PCR 显示非肿瘤性样本中存在 MCPyV-DNA：总数为 41/41（100%）；成人 39/39（100%）；胎儿或婴儿 0/2；男性 28/28（100%）；女性 13/13（100%）；总人体组织 572/572（100%）；皮肤 15/15（100%）；肾上腺 33/33（100%），其他 16 个器官（4-25%）。本研究首次报道了 MCPyV-DNA 存在于甲状腺、肾上腺、脾、骨髓、胃、胆囊、胰腺、心脏和主动脉的非肿瘤性组织中。PCR 显示，与 Merkel 细胞癌样本相比，所有 MCPyV 阳性组织中的病毒载量范围为 0.00026 至 0.22。这些详细的 PCR 数据显示，MCPyV 感染在日本男性中的流行率高于女性（p = 0.004），并且在更多的非肿瘤性人体组织中广泛分布，病毒载量较低，这与之前的报告不同。这些数据为进一步研究 MCPyV 感染和 MCPyV 相关疾病提供了有价值的见解。
