Whittaker B L, Chipley J R
J Environ Pathol Toxicol Oncol. 1986 Mar-Apr;6(3-4):295-304.
Studies were conducted to determine possible interactions between aflatoxin B1 and deoxyribonucleic acid (DNA) and induction of bacteriophage formation in lysogenic Bacillus megaterium NRRL-B-3695. At pH 7.4, the spectrophotometric characteristics of B1 with various concentrations of calf thymus DNA were altered with both a hypochromic shift and a shift of the wavelength for maximum absorbance of the toxin. Aflatoxin B1 apparently interacted with DNA as indicated by spectrophotometric analysis and ultrafiltration studies. Under alkaline conditions (pH 10.0), spectrophotometric characteristics of this interaction were altered from those observed at pH 7.4. Toxin incubated at pH 7.4 could induce bacteriophage formation but failed to do so at pH 10. Preincubation of toxin with calf thymus DNA did not block subsequent induction. It is proposed that a bacterial DNA-toxin interaction, involving an alkaline-labile bond of aflatoxin B1, is necessary to cause induction of bacteriophage formation in lysogenic B. megaterium.
开展了多项研究，以确定黄曲霉毒素B1与脱氧核糖核酸（DNA）之间可能存在的相互作用，以及在溶源性巨大芽孢杆菌NRRL-B-3695中诱导噬菌体形成的情况。在pH 7.4条件下，不同浓度的小牛胸腺DNA与B1的分光光度特性发生了改变，毒素的最大吸光度出现了减色位移和波长位移。分光光度分析和超滤研究表明，黄曲霉毒素B1显然与DNA发生了相互作用。在碱性条件（pH 10.0）下，这种相互作用的分光光度特性与在pH 7.4时观察到的不同。在pH 7.4下孵育的毒素可诱导噬菌体形成，但在pH 10时则不能。毒素与小牛胸腺DNA预孵育不会阻止随后的诱导。有人提出，细菌DNA与毒素的相互作用涉及黄曲霉毒素B1的一个碱不稳定键，这对于在溶源性巨大芽孢杆菌中诱导噬菌体形成是必要的。
