Whittaker B L, Chipley J R
J Environ Pathol Toxicol Oncol. 1986 Mar-Apr;6(3-4):327-37.
The present study was conducted to determine the effects of metabolized aflatoxin B1 (via a mammalian liver mixed-function oxidase system) and native aflatoxin B1 upon induction of bacteriophage in Bacillus subtilis and growth of B. subtilis. A lysogenic strain of B. subtilis (BDS-1, phi 105) and an indicator strain of this species (DBS-1) were utilized in the present experiment. Lysogenic cultures were incubated for various lengths of time in the presence of either native or metabolized toxin, and plaque-forming units were determined. Identical experiments were conducted with the indicator strain and colony-forming units were determined. At a native toxin concentration of 25 micrograms/ml of medium, the maximum number of plaque-forming units was induced in lysogenic cells. However, when cells were incubated in the presence of aflatoxin B1 and mixed-function oxidase, neither plaque-forming units nor colony-forming units could be detected from lysogenic or indicator cells, respectively. This organism is apparently much more susceptible to inhibition of cellular replication than to lysis via bacteriophage induction. Thus, from the present study and from previously reported research, differences in susceptibility to native and metabolized aflatoxin B1 exist among species of Bacillus.
本研究旨在确定代谢型黄曲霉毒素B1（通过哺乳动物肝脏混合功能氧化酶系统）和天然黄曲霉毒素B1对枯草芽孢杆菌中噬菌体诱导及枯草芽孢杆菌生长的影响。本实验使用了枯草芽孢杆菌的溶原性菌株（BDS - 1，phi 105）和该物种的指示菌株（DBS - 1）。将溶原性培养物在天然或代谢毒素存在下孵育不同时间，并测定噬菌斑形成单位。对指示菌株进行相同实验并测定菌落形成单位。在培养基中天然毒素浓度为25微克/毫升时，溶原性细胞中诱导出的噬菌斑形成单位数量最多。然而，当细胞在黄曲霉毒素B1和混合功能氧化酶存在下孵育时，分别从溶原性细胞或指示细胞中均未检测到噬菌斑形成单位或菌落形成单位。该生物体显然对细胞复制抑制比对通过噬菌体诱导的裂解更敏感。因此，从本研究和先前报道的研究来看，枯草芽孢杆菌不同物种对天然和代谢型黄曲霉毒素B1的敏感性存在差异。
