Kobayashi Ikuo, Akita Masaru, Takasu Masaki, Tozaki Teruaki, Kakoi Hironaga, Nakamura Kotono, Senju Natsuko, Matsuyama Ryota, Horii Yoichiro
Sumiyoshi Livestock Science Station, Field Science Center, University of Miyazaki, 10100-1 Shimanouchi, Miyazaki 880-0121, Japan.
Kushima City, 5550 Nishikata, Kushima, Miyazaki 888-8555, Japan.
J Vet Med Sci. 2019 May 11;81(5):707-711. doi: 10.1292/jvms.18-0565. Epub 2019 Mar 12.
The Misaki horse is a Japanese native horse, known as the "feral horse of Cape Toi". In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.
三崎马是日本本土马，被誉为“土井岬野马”。在本研究中，我们获取了用于建立其血统登记簿的遗传信息，并分析了它们的遗传特征以进行保护。我们对77匹三崎马（占种群的80.2%）的32个微卫星和线粒体D环区域进行了基因分型。等位基因的平均数量、观察到的杂合度和预期杂合度分别为3.4、0.509和0.497。构建了个体的邻接法系统发育树。此外，结果表明三崎马经历了瓶颈效应，但并不严重且并非近期发生。此外，确认了三种线粒体单倍型。因此，我们阐明了长期栖息在土井岬的三崎马的遗传背景。
