Nakamura Mako, Tozaki Teruaki, Kakoi Hironaga, Nakamura Kotono, Rajabi-Toustani Reza, Ohba Yasunori, Matsubara Tatsuya, Takasu Masaki
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
J Vet Med Sci. 2020 Apr 15;82(4):503-540. doi: 10.1292/jvms.19-0535. Epub 2020 Mar 9.
The Kiso horse is native to Japan and is on the verge of extinction. Here, we used microsatellites to characterize changes in their genetic diversity over time. We divided a population of Kiso horses that genotyped during 2007-2017 into three groups based on birth year: Group 1, 1980-1998 (70 horses); Group 2, 1999-2007 (61 horses); and Group 3, 2008-2017 (42 horses). We genotyped 31 microsatellites to calculate average number of alleles, observed heterozygosity, and expected heterozygosity. All indicators decreased across age groups. The results indicate that Kiso horses have been experiencing a drop in genetic diversity, and the population is expected to experience further decline unless appropriate measures are implemented.
木曾马原产于日本，现已濒临灭绝。在此，我们使用微卫星来表征其遗传多样性随时间的变化。我们将在2007年至2017年期间进行基因分型的木曾马种群根据出生年份分为三组：第1组，1980 - 1998年（70匹马）；第2组，1999 - 2007年（61匹马）；第3组，2008 - 2017年（42匹马）。我们对31个微卫星进行基因分型，以计算等位基因平均数、观察杂合度和预期杂合度。所有指标在各年龄组中均有所下降。结果表明，木曾马的遗传多样性一直在下降，除非采取适当措施，否则该种群预计还会进一步减少。
