• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences.慢生根瘤菌的nifH和nifDK操纵子的激活取决于启动子上游的DNA序列。
Nucleic Acids Res. 1986 May 27;14(10):4207-27. doi: 10.1093/nar/14.10.4207.
2
Expression of Rhizobium japonicum nifH and nifDK operons can be activated by the Klebsiella pneumonia nifA protein but not by the product of ntrC.日本根瘤菌nifH和nifDK操纵子的表达可被肺炎克雷伯菌nifA蛋白激活，但不能被ntrC的产物激活。
Mol Gen Genet. 1985;199(2):306-14. doi: 10.1007/BF00330273.
3
Construction of chimaeric promoter regions by exchange of the upstream regulatory sequences from fdhF and nif genes.通过交换fdhF和nif基因的上游调控序列构建嵌合启动子区域。
Mol Microbiol. 1989 Jun;3(6):697-703. doi: 10.1111/j.1365-2958.1989.tb00218.x.
4
Identification and characterization of the nifH and nifJ promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerans 333.成团肠杆菌333的固氮质粒pEA3上nifH和nifJ启动子区域的鉴定与表征
Gene. 1989 May 15;78(1):101-9. doi: 10.1016/0378-1119(89)90318-1.
5
Regulation of the fixA gene and fixBC operon in Bradyrhizobium japonicum.日本慢生根瘤菌中fixA基因和fixBC操纵子的调控
J Bacteriol. 1988 Mar;170(3):1205-14. doi: 10.1128/jb.170.3.1205-1214.1988.
6
The nifH and nifDK promoter regions from Rhizobium japonicum share structural homologies with each other and with nitrogen-regulated promoters from other organisms.来自日本根瘤菌的nifH和nifDK启动子区域彼此之间以及与其他生物体中受氮调控的启动子具有结构同源性。
J Mol Appl Genet. 1984;2(4):392-405.
7
A Rhizobium meliloti symbiotic regulatory gene.一个苜蓿根瘤菌共生调控基因。
Cell. 1984 Apr;36(4):1035-43. doi: 10.1016/0092-8674(84)90053-9.
8
Activation of Klebsiella pneumoniae and Rhizobium meliloti nitrogenase promoters by gln (ntr) regulatory proteins.谷氨酰胺（ntr）调节蛋白对肺炎克雷伯菌和苜蓿根瘤菌固氮酶启动子的激活作用。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4030-4. doi: 10.1073/pnas.80.13.4030.
9
The symbiotic nitrogen fixation regulatory operon (fixRnifA) of Bradyrhizobium japonicum is expressed aerobically and is subject to a novel, nifA-independent type of activation.日本慢生根瘤菌的共生固氮调节操纵子（fixRnifA）在有氧条件下表达，并且受到一种新型的、不依赖nifA的激活作用。
Nucleic Acids Res. 1987 Oct 26;15(20):8479-99. doi: 10.1093/nar/15.20.8479.
10
The pleiotropic nature of symbiotic regulatory mutants: Bradyrhizobium japonicum nifA gene is involved in control of nif gene expression and formation of determinate symbiosis.共生调控突变体的多效性本质：日本慢生根瘤菌nifA基因参与固氮基因表达的调控以及定型共生的形成。
EMBO J. 1986 Jun;5(6):1165-73. doi: 10.1002/j.1460-2075.1986.tb04342.x.

引用本文的文献

1
Conserved small mRNA with an unique, extended Shine-Dalgarno sequence.具有独特延伸 Shine-Dalgarno 序列的保守小 mRNA。
RNA Biol. 2017 Oct 3;14(10):1353-1363. doi: 10.1080/15476286.2016.1256534. Epub 2016 Nov 11.
2
Genome-wide transcription start site mapping of Bradyrhizobium japonicum grown free-living or in symbiosis - a rich resource to identify new transcripts, proteins and to study gene regulation.日本慢生根瘤菌在自由生活或共生状态下的全基因组转录起始位点定位——这是鉴定新转录本、蛋白质以及研究基因调控的丰富资源。
BMC Genomics. 2016 Apr 23;17:302. doi: 10.1186/s12864-016-2602-9.
3
A vector for the site-directed, genomic integration of foreign DNA into soybean root-nodule bacteria.大豆根瘤菌中外源 DNA 定点基因组整合的载体。
Plant Mol Biol. 1987 Jan;9(1):41-50. doi: 10.1007/BF00017985.
4
Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae.用于豌豆根瘤菌生物型蚕豆中氢化酶基因启动子的NifA依赖性激活的增强子序列的新排列。
J Bacteriol. 2008 May;190(9):3185-91. doi: 10.1128/JB.00107-08. Epub 2008 Feb 29.
5
Dissection of the Bradyrhizobium japonicum NifA+sigma54 regulon, and identification of a ferredoxin gene (fdxN) for symbiotic nitrogen fixation.慢生根瘤菌NifA+σ54调控子的剖析以及共生固氮铁氧化还原蛋白基因（fdxN）的鉴定。
Mol Genet Genomics. 2007 Sep;278(3):255-71. doi: 10.1007/s00438-007-0246-9. Epub 2007 Jun 15.
6
Direct response of Bradyrhizobium japonicum nifA-mediated nif gene regulation to cellular oxygen status.日本慢生根瘤菌nifA介导的nif基因调控对细胞氧状态的直接响应。
Mol Gen Genet. 1987 Oct;209(3):621-6. doi: 10.1007/BF00331174.
7
The Iron control element, acting in positive and negative control of iron-regulated Bradyrhizobium japonicum genes, is a target for the Irr protein.铁调控元件对日本慢生根瘤菌的铁调节基因发挥正调控和负调控作用，是Irr蛋白的作用靶点。
J Bacteriol. 2006 Jan;188(2):733-44. doi: 10.1128/JB.188.2.733-744.2006.
8
Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans.固氮类芽孢杆菌中三个nifH同源基因的系统发育及特性分析
Appl Environ Microbiol. 2003 Jun;69(6):3658-62. doi: 10.1128/AEM.69.6.3658-3662.2003.
9
In vivo genomic footprinting analysis reveals that the complex Bradyrhizobium japonicum fixRnifA promoter region is differently occupied by two distinct RNA polymerase holoenzymes.体内基因组足迹分析表明，复杂的日本慢生根瘤菌fixRnifA启动子区域被两种不同的RNA聚合酶全酶以不同方式占据。
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1014-9. doi: 10.1073/pnas.95.3.1014.
10
The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis.慢生根瘤菌的乌头酸酶基因（acnA）对其自由生活生长很重要，但对有效的根瘤共生并不重要。
J Bacteriol. 1996 Nov;178(21):6166-72. doi: 10.1128/jb.178.21.6166-6172.1996.

本文引用的文献

1
Azotobacter vinelandii nifD- and nifE-encoded polypeptides share structural homology.维氏固氮菌 nifD 和 nifE 编码的多肽具有结构同源性。
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5720-3. doi: 10.1073/pnas.82.17.5720.
2
Nitrogenase reductase: A functional multigene family in Rhizobium phaseoli.固氮酶还原酶：根瘤菌属中的一个功能性多基因家族。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1170-4. doi: 10.1073/pnas.82.4.1170.
3
Structural genes of dinitrogenase and dinitrogenase reductase are transcribed from two separate promoters in the broad host range cowpea Rhizobium strain IRc78.固氮酶和固氮酶还原酶的结构基因由广宿主范围豇豆根瘤菌菌株 IRc78 的两个独立启动子转录。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7358-62. doi: 10.1073/pnas.81.23.7358.
4
Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti.苜蓿中华根瘤菌调控固氮基因（fixD）的定位与表达。
EMBO J. 1985 Nov;4(11):2751-6. doi: 10.1002/j.1460-2075.1985.tb03999.x.
5
Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene.根瘤菌共生区的克隆：结瘤基因位于固氮酶基因和一个类似于 nifA 的基因之间。
EMBO J. 1983;2(6):947-52. doi: 10.1002/j.1460-2075.1983.tb01526.x.
6
Deletion analysis of Rhizobium meliloti symbiotic promoters.苜蓿根瘤菌共生启动子的缺失分析
EMBO J. 1985 Oct;4(10):2419-24. doi: 10.1002/j.1460-2075.1985.tb03950.x.
7
Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.肺炎克雷伯菌中固氮基因簇的氮调控：ntrA基因的作用以及ntrC与nifA之间的相似性
EMBO J. 1983;2(1):39-44. doi: 10.1002/j.1460-2075.1983.tb01377.x.
8
An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.一个用于比较和比对核酸及氨基酸序列的交互式图形程序。
Nucleic Acids Res. 1982 May 11;10(9):2951-61. doi: 10.1093/nar/10.9.2951.
9
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51. doi: 10.1073/pnas.77.12.7347.
10
Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. pneumoniae.肺炎克雷伯菌中参与固氮酶合成氧调节的固氮基因（nifL）。
Nature. 1981 Apr 2;290(5805):424-6. doi: 10.1038/290424a0.

慢生根瘤菌的nifH和nifDK操纵子的激活取决于启动子上游的DNA序列。

Activation of the Bradyrhizobium japonicum nifH and nifDK operons is dependent on promoter-upstream DNA sequences.

作者信息

Alvarez-Morales A, Betancourt-Alvarez M, Kaluza K, Hennecke H

出版信息

Nucleic Acids Res. 1986 May 27;14(10):4207-27. doi: 10.1093/nar/14.10.4207.

DOI:10.1093/nar/14.10.4207
PMID:3086837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339856/
Abstract

Previous analysis of B. japonicum nifH'- and nifD'-'lacZ translational fusions showed that these promoters could be activated by the K. pneumoniae nifA plus the E. coli ntrA gene products. To study the functions of the DNA 5' to these promoters, plasmids carrying deletions in this region were constructed and analyzed in vivo in a heterologous system consisting of an E. coli (NtrA+) background with a plasmid that constitutively expresses the K. pneumoniae nifA gene. Activation of the B. japonicum promoters was completely dependent on sequences located between positions -165 and -100, relative to the start of transcription. Some of the nifD deletion-fusions were mobilized to the wild-type B. japonicum and the exconjugants tested in an ex planta micro-aerobic system, and also used to infect soybean seedlings. The time course of derepression was followed by assaying beta-galactosidase activity from samples withdrawn from the microaerobic cultures or from root-nodule extracts. The results conclusively show that in the homologous system the sequences upstream of the promoter are required to achieve wild-type activity.

摘要

先前对日本固氮菌nifH'-和nifD'-'lacZ翻译融合体的分析表明，这些启动子可被肺炎克雷伯菌nifA加上大肠杆菌ntrA基因产物激活。为了研究这些启动子5'端DNA的功能，构建了在该区域带有缺失的质粒，并在由表达肺炎克雷伯菌nifA基因的质粒和大肠杆菌（NtrA+）背景组成的异源系统中进行体内分析。日本固氮菌启动子的激活完全依赖于相对于转录起始位置-165至-100之间的序列。一些nifD缺失融合体被转移到野生型日本固氮菌中，对接合后体在植物外微需氧系统中进行测试，并用于感染大豆幼苗。通过检测从微需氧培养物或根瘤提取物中取出的样品的β-半乳糖苷酶活性来跟踪去阻遏的时间进程。结果确凿地表明，在同源系统中，启动子上游的序列是实现野生型活性所必需的。