Greif Gonzalo, Berná Luisa, Díaz-Viraqué Florencia, Robello Carlos
Laboratory of Host Pathogen Interactions-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Departamento de Bioquímica, Facultad de Medicina-UDELAR, Montevideo, Uruguay.
Methods Mol Biol. 2019;1955:35-45. doi: 10.1007/978-1-4939-9148-8_3.
RNA-seq is a powerful method to study in detail transcriptome changes in defined conditions, providing enormous amount of information on RNA stability and gene regulation. In this chapter, we describe a directional and a nondirectional library preparation protocol for RNA-seq in Trypanosoma cruzi, as well as a pipeline for bioinformatic analysis, which includes read trimming, alignment to a reference genome, and differential expression.
RNA测序是一种在特定条件下详细研究转录组变化的强大方法，可提供有关RNA稳定性和基因调控的大量信息。在本章中，我们描述了用于克氏锥虫RNA测序的定向和非定向文库制备方案，以及生物信息学分析流程，其中包括读段修剪、与参考基因组比对和差异表达分析。
