Carlevaro Giannina, Lantos Andrés B, Cánepa Gaspar E, de Los Milagros Cámara María, Somoza Martín, Buscaglia Carlos A, Campetella Oscar, Mucci Juan
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Methods Mol Biol. 2019;1955:135-146. doi: 10.1007/978-1-4939-9148-8_10.
Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface glycoconjugates from host target cells) by means of a unique trans-sialidase (TS) enzyme. TS-induced changes in the glycoprotein sialylation profile of both parasite and host cells are crucial for the establishment of a persistent T. cruzi infection and for the development of Chagas disease-associated pathogenesis. In this chapter, we describe a novel metabolic labeling method developed in our labs that enables straightforward identification and molecular characterization of SA acceptors of the TS-catalyzed reaction.
克氏锥虫是恰加斯病的原生动物病原体，它进化出了一种创新的代谢途径，通过这种途径，保护性唾液酸（SA）残基从宿主唾液酸糖缀合物中被清除，并通过一种独特的转唾液酸酶（TS）转移到寄生虫表面的黏蛋白样分子（或宿主靶细胞的表面糖缀合物）上。TS诱导的寄生虫和宿主细胞糖蛋白唾液酸化谱的变化对于克氏锥虫持续性感染的建立以及恰加斯病相关发病机制的发展至关重要。在本章中，我们描述了我们实验室开发的一种新型代谢标记方法，该方法能够直接鉴定TS催化反应的SA受体并对其进行分子表征。
