Leguizamón María Susana
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Methods Mol Biol. 2019;1955:239-246. doi: 10.1007/978-1-4939-9148-8_18.
The trans-sialidase (TS), a virulence factor expressed on the surface of Trypanosoma cruzi, the agent of Chagas disease, is an enzyme that transfers sialic acids between glycoconjugates. In humans and most tested mammals, the onset of the chronic phase of T. cruzi infection correlates with the elicitation of antibodies directed to the TS catalytic domain, which inhibit the sialyl residues transfer reaction in vitro and in vivo. The method described here, termed trans-sialidase inhibition assay (TIA), enables the detection of TS-neutralizing antibodies in serum samples of different mammalian species, without the use of conjugated secondary reagents. The high specificity and exquisite sensitivity displayed by the TIA allow to overcome the limitations of routinely used Chagas disease serodiagnostic assays.
转唾液酸酶（TS）是恰加斯病病原体克氏锥虫表面表达的一种毒力因子，是一种在糖缀合物之间转移唾液酸的酶。在人类和大多数经过检测的哺乳动物中，克氏锥虫感染慢性期的开始与针对TS催化结构域的抗体的诱导有关，这些抗体在体外和体内均可抑制唾液酸残基转移反应。此处所述的方法称为转唾液酸酶抑制试验（TIA），无需使用偶联的二抗试剂，即可检测不同哺乳动物物种血清样本中的TS中和抗体。TIA显示出的高特异性和极高灵敏度使其能够克服常规使用的恰加斯病血清诊断试验的局限性。
