Albareda María Cecilia, Natale María Ailén, Cesar Gonzalo Leandro, Castro Eiro Melisa Daiana, Alvarez María Gabriela, Laucella Susana Adriana
Instituto Nacional de Parasitología Dr. M. Fatala Chaben, Buenos Aires, Argentina.
Hospital Interzonal General de Agudos Eva Perón, Buenos Aires, Argentina.
Methods Mol Biol. 2019;1955:339-348. doi: 10.1007/978-1-4939-9148-8_25.
Chagas disease is the highest impact parasitic disease in Latin America. In recent years, the use of immune-related biomarkers to predict diagnostic and treatment efficacy or to monitor diseases has been considered a promising tool. Our group has worked for the past 20 years on the characterization of different immunological aspects of the T-cell responses to T. cruzi antigens. We have shown that monitoring of appropriate immunological responses can provide earlier and robust measures of treatment.The Enzyme-Linked ImmunoSPOT (ELISPOT) assays are powerful tools to evaluate antigen-specific immune responses at the single-cell level. Herein, we describe uses of the ELISPOT assay to determine the T. cruzi-specific T-cell populations in PBMCs from chronic chagasic subjects.
恰加斯病是拉丁美洲影响最大的寄生虫病。近年来，使用免疫相关生物标志物来预测诊断和治疗效果或监测疾病被认为是一种很有前景的工具。在过去20年里，我们团队致力于表征针对克氏锥虫抗原的T细胞反应的不同免疫学方面。我们已经表明，监测适当的免疫反应可以提供更早且有力的治疗指标。酶联免疫斑点（ELISPOT）检测是在单细胞水平评估抗原特异性免疫反应的强大工具。在此，我们描述了ELISPOT检测在确定慢性恰加斯病患者外周血单核细胞中克氏锥虫特异性T细胞群体方面的应用。
