Lasso Paola, Mateus Jose, González John Mario, Cuéllar Adriana, Puerta Concepción
Grupo de Inmunobiología y Biología Celular, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Grupo de Enfermedades Infecciosas, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Methods Mol Biol. 2019;1955:349-361. doi: 10.1007/978-1-4939-9148-8_26.
Flow cytometry is a valuable technique in cellular immunology that allows evaluating effective parameters of the immune response associated with CD8 T cells. During Chagas disease, infection caused by Trypanosoma cruzi parasite, similar to other intracellular infectious agents, antigen-specific CD8 T cells are essential for controlling the infection. However, CD8 T cell response is only partially effective in some chronic Chagas disease patients. Thus, characterization and phenotyping of T. cruzi-specific CD8 T cells are of great importance during chronic Chagas disease.
流式细胞术是细胞免疫学中的一项重要技术，可用于评估与CD8 T细胞相关的免疫反应有效参数。在恰加斯病（由克氏锥虫寄生虫引起的感染）期间，与其他细胞内感染因子类似，抗原特异性CD8 T细胞对于控制感染至关重要。然而，在一些慢性恰加斯病患者中，CD8 T细胞反应仅部分有效。因此，在慢性恰加斯病期间，对克氏锥虫特异性CD8 T细胞进行表征和表型分析非常重要。
