Department of Thoracic Surgery, School of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
Department of Thoracic Surgery, Private Medstar Antalya Hospital, Antalya, Turkey.
Curr Mol Med. 2019;19(3):216-227. doi: 10.2174/1566524019666190314113800.
We aimed at exploring biological functions of differentially expressed miRNAs during carcinogenesis, to identify miRNAs dysegulations involved in DNA repair mechanisms, and to evaluate potential of miRNAs as prognostic and diagnostic biomarkers for early lung adenocarcinomas (LAC).
We obtained 21 LAC and paired adjacent normal formalin-fixed, paraffinembedded lung tissues from patients who underwent curative resection for stage I LAC. We compared expression levels of eight miRNAs involved in the DNA repair mechanism between LAC and adjacent tissues.
Expressions of Hsa-miR-9-5p, hsa-miR-24-3p, hsa-miR-125a-3p, hsa-miR- 125b-5p, hsa-miR-155-5p, and hsa-let-7a-5p were significantly up-regulated in stage I LAC tissues compared with those in the adjacent tissues. In addition, expressions of hsa-mir-9-5p, hsa-mir-24-3p, hsa-mir-125a-3p, hsa-mir-125b-5p, and hsa-mir-155-5p were significantly up-regulated in stage Ia LAC tissues, whereas expressions of hsa-mir- 125a-3p and hsa-mir-125b-5p were significantly up-regulated in stage Ib LAC tissues. Receiver operating characteristic (ROC) analysis revealed that AUROC of hsa-mir-125b- 5p was 0.875 (P < 0.001).
Expression of hsa-mir-125b-5p could be used to distinguish LAC from adjacent tissues. Our result suggests that hsa-mir125b-5p can be a prognostic and diagnostic biomarker for LAC.
本研究旨在探讨差异表达 miRNA 在癌变过程中的生物学功能，寻找与 DNA 修复机制相关的 miRNA 失调，评估 miRNA 作为早期肺腺癌（LAC）的预后和诊断生物标志物的潜力。
本研究收集了 21 例接受 I 期 LAC 根治性切除术的患者的 21 例 LAC 及配对的相邻正常福尔马林固定、石蜡包埋肺组织。比较了 LAC 与相邻组织中 8 种参与 DNA 修复机制的 miRNA 的表达水平。
与相邻组织相比，I 期 LAC 组织中 hsa-miR-9-5p、hsa-miR-24-3p、hsa-miR-125a-3p、hsa-miR-125b-5p、hsa-miR-155-5p 和 hsa-let-7a-5p 的表达显著上调。此外，hsa-mir-9-5p、hsa-mir-24-3p、hsa-mir-125a-3p、hsa-mir-125b-5p 和 hsa-mir-155-5p 在 Ia 期 LAC 组织中表达显著上调，而 hsa-mir-125a-3p 和 hsa-mir-125b-5p 在 Ib 期 LAC 组织中表达显著上调。受试者工作特征（ROC）分析显示 hsa-mir-125b-5p 的 AUROC 为 0.875（P<0.001）。
hsa-mir-125b-5p 的表达可用于区分 LAC 与相邻组织。我们的结果表明 hsa-mir-125b-5p 可作为 LAC 的预后和诊断生物标志物。
