新月柄杆菌中 Hfq 结合 RNA 的鉴定。
Identification of Hfq-binding RNAs in Caulobacter crescentus.
机构信息
a Departamento de Microbiologia, Instituto de Ciências Biomédicas , Universidade de São Paulo , São Paulo , Brazil.
b Biozentrum , University of Basel , Basel , Switzerland.
出版信息
RNA Biol. 2019 Jun;16(6):719-726. doi: 10.1080/15476286.2019.1593091. Epub 2019 Mar 26.
Abstract
Small RNAs are important for post-transcriptional regulation of gene expression, affecting stability and activity of their target mRNAs. The bacterial Sm-like protein Hfq is required to promote pairing between both RNAs when their sequence complementarity is limited. To provide a first global view on the post-transcriptional landscape of the α-proteobacterium Caulobacter crescentus, we have identified the Hfq-binding RNAs employing High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP). A total of 261 RNAs, including 3 unannotated RNAs, were successfully identified and classified according to putative function. Moreover, possible interactions between the identified sRNAs with mRNA targets were postulated through computational target predictions.
摘要
小 RNA 对于基因表达的转录后调控非常重要，影响其靶 mRNA 的稳定性和活性。当 RNA 序列互补性有限时，细菌 Sm 样蛋白 Hfq 对于促进两者之间的配对是必需的。为了首次提供α变形菌新月柄杆菌转录后景观的全局视图，我们通过交联免疫沉淀（HITS-CLIP）分离的 RNA 进行高通量测序，鉴定了 Hfq 结合的 RNA。总共鉴定出 261 个 RNA，包括 3 个未注释的 RNA，根据推测的功能进行了分类。此外，通过计算靶预测，提出了所鉴定的 sRNA 与 mRNA 靶之间可能的相互作用。
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