常规短暂性肢体缺血可预防高胆固醇血症兔动脉粥样硬化进展。
Regular transient limb ischemia prevents atherosclerosis progression in hypercholesterolemic rabbits.
机构信息
Department of Anesthesia, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China.
Department of Urology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China.
出版信息
Chin Med J (Engl). 2019 May 5;132(9):1079-1086. doi: 10.1097/CM9.0000000000000204.
BACKGROUND
Endothelial dysfunction, the initial pathogenic factor in atherosclerosis, can be alleviated via transient limb ischemia. We observed the effects of regular transient limb ischemia (RTLI) on atherosclerosis in hypercholesterolemic rabbits.
METHODS
Twenty-eight rabbits were randomized to control, cholesterol, sham, ischemia groups (n = 7 each) between October 2010 and March 2011. They were fed a normal diet in the control group and hypercholesterolemic diet in other groups for 12 weeks. Six cycles of RTLI were performed once per day on the ischemia group. Serum samples were prepared to measure the total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) before the experiment (W0), at the end of weeks 4, 8, 12 (W4, W8, W12). The whole aorta was harvested at W12 and stained using Sudan IV to identify the plaque. The plaque area was measured using Image J. Results were analyzed by analysis of variance or rank sum test.
RESULTS
Concentrations of TC in the cholesterol group were higher than those in the control group at W4 (29.60 [23.75, 39.30] vs. 1.00 [0.80, 1.55], Z = -2.745, P = 0.006), W8 (41.78 [28.08, 47.37] vs. 0.35 [0.10, 0.68], Z = -2.739, P = 0.006), W12 (48.32 [40.04, 48.95] vs. 0.61 [0.50, 0.86], Z = -2.739, P = 0.006). Similar results were obtained for HDL-C and LDL-C. Serum concentrations of TC, HDL-C, and LDL-C in the hypercholesterolemic groups had no differences (all P > 0.05). The percentage of plaque area in the cholesterol group was higher than that in the control group (47.22 ± 23.89% vs. 0, Z = -2.986, P = 0.003). Square root of the percentage of plaque area was smaller in the ischemia group than that in the cholesterol (0.44 ± 0.13 vs. 0.67 ± 0.18, P = 0.014) or sham groups (0.44 ± 0.13 vs. 0.61 ± 0.12, P = 0.049).
CONCLUSION
In hypercholesterolemic rabbits, RTLI might prevent atherosclerosis progression by reducing the percentage of plaque area.
背景
内皮功能障碍是动脉粥样硬化的初始致病因素，可以通过短暂肢体缺血得到缓解。我们观察了常规短暂肢体缺血（RTLI）对高胆固醇血症兔动脉粥样硬化的影响。
方法
2010 年 10 月至 2011 年 3 月期间，28 只兔子被随机分为对照组、胆固醇组、假手术组和缺血组（每组 7 只）。对照组给予正常饮食，其他组给予高胆固醇饮食 12 周。缺血组每天进行 6 个周期的 RTLI。在实验前（W0）、第 4、8、12 周末（W4、W8、W12）采集血清样本，测定总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）。在 W12 时取整个主动脉，苏丹 IV 染色鉴定斑块，用 Image J 测量斑块面积。结果采用方差分析或秩和检验进行分析。
结果
胆固醇组在 W4（29.60[23.75, 39.30] vs. 1.00[0.80, 1.55]，Z=-2.745，P=0.006）、W8（41.78[28.08, 47.37] vs. 0.35[0.10, 0.68]，Z=-2.739，P=0.006）、W12（48.32[40.04, 48.95] vs. 0.61[0.50, 0.86]，Z=-2.739，P=0.006）时 TC 浓度高于对照组。HDL-C 和 LDL-C 也有类似结果。高胆固醇血症各组 TC、HDL-C 和 LDL-C 血清浓度无差异（均 P>0.05）。胆固醇组斑块面积百分比高于对照组（47.22±23.89% vs. 0，Z=-2.986，P=0.003）。缺血组斑块面积百分比的平方根小于胆固醇组（0.44±0.13 vs. 0.67±0.18，P=0.014）或假手术组（0.44±0.13 vs. 0.61±0.12，P=0.049）。
结论
在高胆固醇血症兔中，RTLI 可能通过减少斑块面积百分比来预防动脉粥样硬化进展。
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