Department of Herbal Pharmacology, College of Korean Medicine, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Korea.
Korea Institute of Science and Technology for Eastern Medicine (KISTEM) NeuMed Inc., 88 Imun-ro, Dongdaemun-gu, Seoul 02440, Korea.
Int J Mol Sci. 2019 Mar 12;20(5):1253. doi: 10.3390/ijms20051253.
Eleutherococcus extract mixture (EEM) is an herbal mixture of dried stem of and germinated barley, which has been highly effective, in previous screening and among the traditional medicines to tonify innate and acquired , respectively. In this study, we investigate the effects of EEM on endochondral bone formation. Female adolescent rats were given EEM, growth hormone or vehicle for 10 days. Tetracycline was intraperitoneally injected to light the fluorescent band 72 h before sacrifice to determine endochondral bone formation. In order to evaluate endocrine or paracrine/autocrine mechanisms, expressions of insulin-like growth factor 1 (IGF1), insulin-like growth factor binding protein 3 (IGFBP3), or bone morphogenetic protein 2 (BMP2) were evaluated after EEM administration in liver or growth plate (GP). EEM oral administration significantly increased endochondral bone formation and proliferative and hypertrophic zonal heights of tibial GP. EEM also upregulated hepatic IGF1 and IGFBP3 mRNA expressions, and IGF1 and BMP2 expressions in GP. Taken together, EEM increases endochondral bone formation through stimulating proliferation and hypertrophy with upregulation of hepatic IGF1 and IGFBP3 expressions. Considering immunohistochemical studies, the effect of EEM may be due to increased local IGF1 and BMP2 expression in GP, which may be considered growth hormone (GH)-dependent endocrine and autocrine/paracrine pathways.
刺五加提取物混合物（EEM）是一种草药混合物，由干茎和发芽的大麦组成，在之前的筛选和传统医学中，它分别被证明对先天和后天的具有高度有效性。在这项研究中，我们研究了 EEM 对软骨内骨形成的影响。将 EEM、生长激素或载体给予雌性青春期大鼠 10 天。在牺牲前 72 小时，通过腹腔内注射四环素来照亮荧光带，以确定软骨内骨形成。为了评估内分泌或旁分泌/自分泌机制，在给予 EEM 后评估肝脏或生长板（GP）中胰岛素样生长因子 1（IGF1）、胰岛素样生长因子结合蛋白 3（IGFBP3）或骨形态发生蛋白 2（BMP2）的表达。EEM 口服给药显著增加了软骨内骨形成以及胫骨 GP 的增殖和肥大区带的高度。EEM 还上调了肝脏 IGF1 和 IGFBP3 mRNA 的表达，以及 GP 中的 IGF1 和 BMP2 表达。综上所述，EEM 通过刺激增殖和肥大以及上调肝脏 IGF1 和 IGFBP3 的表达来增加软骨内骨形成。考虑到免疫组织化学研究，EEM 的作用可能是由于 GP 中局部 IGF1 和 BMP2 表达增加，这可能被认为是生长激素（GH）依赖性内分泌和自分泌/旁分泌途径。
