Department of Herbal Pharmacology, College of Korean Medicine, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea.
Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea.
J Ethnopharmacol. 2019 May 23;236:108-113. doi: 10.1016/j.jep.2019.01.048. Epub 2019 Mar 5.
According to traditional Korean medicine theory in which children's growth retardation is attributed to blood deficiency, Siwu decoction (SWD), a representative treatment for blood deficiency, was chosen as a sample.
To evaluate the effects of SWD on chondrocyte proliferation of growth plate in adolescent female rats.
Female adolescent rats were allocated to one of the following four groups; SWD 100 and 300 mg/kg, recombinant human growth hormone, and vehicle for 4 days. Tetracycline was intraperitoneally injected at 48 h before sacrifice to obtain a band exhibiting fluorescence by binding newly formed bone. Bromodeoxyuridine was injected at day 2-4 to mark proliferating chondrocytes. To evaluate possible mechanisms of SWD, expressions of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) in the growth plate were examined by immunohistochemistry.
Treatment with SWD significantly increased the number of BrdU-positive chondrocytes and the new bone formation in the proximal growth plate of tibia compared to the vehicle treated control group. SWD also increased the expression of IGF-1 and BMP-2 in the proliferative and hypertrophic zones of the growth plate.
SWD 300 mg/kg stimulates chondrocyte proliferation and new bone formation in the growth plate. Immunohistochemical studies indicate that the effects of SWD may be due to upregulation of local IGF-1 and BMP-2 expression in the growth plate, which may be considered as a GH-dependent paracrine-autocrine pathway.
根据传统的韩国医学理论，儿童生长迟缓归因于血虚，因此选择四物汤（SWD）作为血虚的代表性治疗方法。
评估四物汤对青春期雌性大鼠生长板软骨细胞增殖的影响。
将雌性青春期大鼠分为以下四组之一；SWD 100 和 300mg/kg、重组人生长激素和载体，共 4 天。在处死前 48 小时，通过腹腔内注射四环素以获得与新形成的骨骼结合后显示荧光的条带。在第 2-4 天注射溴脱氧尿苷以标记增殖的软骨细胞。为了评估 SWD 的可能机制，通过免疫组织化学检查生长板中胰岛素样生长因子-1（IGF-1）和骨形态发生蛋白-2（BMP-2）的表达。
与对照组相比，SWD 治疗显著增加了胫骨近端生长板中 BrdU 阳性软骨细胞的数量和新骨形成。SWD 还增加了生长板增殖和肥大区 IGF-1 和 BMP-2 的表达。
SWD 300mg/kg 刺激生长板中的软骨细胞增殖和新骨形成。免疫组织化学研究表明，SWD 的作用可能是由于生长板中局部 IGF-1 和 BMP-2 表达的上调，这可以被认为是一种 GH 依赖的旁分泌-自分泌途径。
