Department of Applied Life Science, Konkuk University, Seoul 143-701, Korea.
Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, Korea.
Molecules. 2019 Mar 13;24(6):1012. doi: 10.3390/molecules24061012.
The genetic diversity and antioxidant potential of L. accessions collected from different geo-ecological regions of South Korea were evaluated and compared. Antioxidant potential of seeds was estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging assays and total phenolic content was determined by the Folin⁻Ciocalteu method. Total phenolic content (TPC) in 80% methanolic extracts ranged from 16.24 ± 0.86 to 58.04 ± 1.00 mg gallic acid equivalent (GAE)/g of the sample extracts and total flavonoid content (TFC) varied from 7.19 ± 1.05 to 52.56 ± 1.50 mg quercetin equivalents (QE) mg/g of the sample extracts. DPPH radical scavenging capacity of the extracts from the 15 accessions of varied from 206.44 ± 7.72 to 2490.24 ± 4.641 mg GAE/g of the sample extracts and ABTS radical scavenging capacity ranged from 624.85 ± 13.1 to 1087. 77 ± 9.58 mg GAE/g of the sample extracts. A wide range of genetic variation was observed as measured by Shannon's information index (I), number of effective alleles (Ne), number of observed alleles (Na), expected heterozygosity (He), unbiased expected heterozygosity (uHe). The observed variation in the bioactive properties, morphological traits, and genetic diversity among the accessions may provide useful information for breeding programs seeking to improve bioactive properties of .
对从韩国不同地理生态区收集的 L. 品系进行了遗传多样性和抗氧化潜力评估,并进行了比较。通过 1,1-二苯基-2-苦基肼基(DPPH)和 2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除测定法评估种子的抗氧化潜力,并通过 Folin⁻Ciocalteu 法测定总酚含量。80%甲醇提取物中的总酚含量(TPC)范围为 16.24±0.86 至 58.04±1.00 mg 没食子酸当量(GAE)/g 样品提取物,总类黄酮含量(TFC)范围为 7.19±1.05 至 52.56±1.50 mg 槲皮素当量(QE)mg/g 样品提取物。15 个 L. 品系提取物的 DPPH 自由基清除能力范围为 206.44±7.72 至 2490.24±4.641 mg GAE/g 样品提取物,ABTS 自由基清除能力范围为 624.85±13.1 至 1087.77±9.58 mg GAE/g 样品提取物。通过香农信息指数(I)、有效等位基因数(Ne)、观察等位基因数(Na)、期望杂合度(He)和无偏期望杂合度(uHe)测量,观察到遗传变异范围广泛。品系间生物活性特性、形态特征和遗传多样性的观察变异可能为寻求提高生物活性特性的 育种计划提供有用信息。