Department of Applied Life Science, Konkuk University, Seoul 143-701, South Korea.
Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, South Korea.
Plant Physiol Biochem. 2017 Sep;118:77-87. doi: 10.1016/j.plaphy.2017.06.006. Epub 2017 Jun 7.
This study evaluated the effects of enhanced concentrations of α-tocopherol and phenolic compounds on the resistance and stability of Perilla oil in transgenic Perilla frutescens plants against various tested pathogenic bacteria by over-expressing the γ-tmt gene. The concentration of phenolic compounds in the non-transgenic samples was 9313.198 ± 18.887 μg g dry weight (DW), whereas the total concentration of the transgenic samples ranged from 9118.015 ± 18.822 to 10527.612 ± 20.411 μg g DW. The largest increases in phenolic compounds in the transgenic plants in comparison with the control plants were observed in gallic acid, pyrogallol, 5-sulfosalicylic acid, catechin, chlorogenic acid, vanillin, syringic acid, naringenin, salicylic acid, quercetin, o-coumaric acid, kaempferol, and hesperetin. o-coumaric and benzoic acid acid were the most abundant phenolic acids found in the transgenic plants. Gram-negative bacteria (Salmonella typhimurium) were the most susceptible microorganism against transgenic ethyl acetate extracts with lower measurement of minimum inhibitory concentration (MICs) (0.25 ± 0.03 mg/ml) at an extract concentration of 2 mg/ml in dried plant material. The same extracts were more effective against gram-positive bacteria (Bacillus subtilis) when compared to control plants with MICs values of 0.52 ± 0.02 mg/ml. The suplementation of 20 μg of α-tocopherol (1000 ppm) in combination with ethyl acetate extracts enhanced the antimicrobial activity against S. typhimurium and B. subtilis, compared to the non-transgenic plants. The acid value of transgenic Perilla oil improved by 91.2% and 35.54% relative to the non-transgenic control oil and commercial Perilla oil, respectively. The low acid value suggests that the oil will be less susceptible to lipase action, and more economically viable and thus, may also improve the oil quality for industrial purposes. In addition, extracts obtained from transgenic plants could be a potential source of antimicrobial agents for the treatment of bacterial infections.
本研究通过过表达γ-tmt 基因，评估了增强的α-生育酚和酚类化合物浓度对转基因为紫苏的植物中紫苏油的抗性和稳定性的影响，以抵抗各种测试的病原菌。非转基因样品中的酚类化合物浓度为 9313.198±18.887μg/g 干重（DW），而转基因样品的总浓度范围为 9118.015±18.822 至 10527.612±20.411μg/g DW。与对照植物相比，转基因植物中酚类化合物的最大增加量出现在没食子酸、焦儿茶酚、5-磺基水杨酸、儿茶素、绿原酸、香草醛、丁香酸、柚皮苷、水杨酸、槲皮素、邻香豆酸、山奈酚和橙皮素。邻香豆酸和苯甲酸是转基因植物中含量最丰富的酚酸。革兰氏阴性菌（鼠伤寒沙门氏菌）是最易受影响的微生物，对浓度为 2mg/ml 干植物材料的转基因乙酸乙酯提取物的最小抑菌浓度（MICs）（0.25±0.03mg/ml）测量值最低。与对照植物相比，相同提取物对革兰氏阳性菌（枯草芽孢杆菌）更有效，MIC 值为 0.52±0.02mg/ml。与非转基因植物相比，20μg α-生育酚（1000ppm）的补充与乙酸乙酯提取物一起增强了对 S.typhimurium 和 B.subtilis 的抗菌活性。与非转基因对照油和商业紫苏油相比，转基因紫苏油的酸值提高了 91.2%和 35.54%。低酸值表明油将不易受到脂肪酶的作用，并且更具经济可行性，因此也可能提高工业用途的油质量。此外，从转基因植物中获得的提取物可能是治疗细菌感染的潜在抗菌剂来源。
