大肠杆菌中OmpC和OmpF对碳青霉烯类压力差异梯度的转录反应。
Transcriptional response of OmpC and OmpF in Escherichia coli against differential gradient of carbapenem stress.
作者信息
Chetri Shiela, Singha Melson, Bhowmik Deepshikha, Nath Kathakali, Chanda Debadatta Dhar, Chakravarty Atanu, Bhattacharjee Amitabha
机构信息
Department of Microbiology, Assam University, Silchar, Assam, India.
Department of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India.
出版信息
BMC Res Notes. 2019 Mar 14;12(1):138. doi: 10.1186/s13104-019-4177-4.
OBJECTIVE
This study was designed to investigate the transcriptional response of OmpF and OmpC along with an antisense RNA, MicF under concentration gradient carbapenem exposure.
RESULT
An elevation in the expression of OmpF gene under concentration gradient imipenem stress from a particular concentration was observed. For OmpC gene a significant decrease in the expression was noticed under concentration gradient imipenem and meropenem stress. The study showed reduction in the expression of OmpC gene against imipenem and meropenem possibly preventing the entry of carbapenem antibiotic inside the cell indicating a possible role in carbapenem resistance.
目的
本研究旨在调查在浓度梯度碳青霉烯暴露下，外膜蛋白F（OmpF）、外膜蛋白C（OmpC）以及一种反义RNA，即微小RNA（MicF）的转录反应。
结果
在特定浓度的亚胺培南浓度梯度应激下，观察到OmpF基因表达升高。对于OmpC基因，在亚胺培南和美罗培南浓度梯度应激下，其表达显著降低。该研究表明，针对亚胺培南和美罗培南，OmpC基因表达降低，可能阻止了碳青霉烯抗生素进入细胞，提示其在碳青霉烯耐药中可能发挥作用。
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