Zimin Iu I, Cheredeev A N, Novikov V I, Vlasov A A
Tsitologiia. 1978 Jun;20(6):690-4.
T--enriched lymphocyte fraction from normal donors was isolated from heparinized peripheral blood on Ficoll-Urotrast gradients. The mitogenic response of T-lymphocytes in culture was 3 times as high as that of unseparated mononuclear cells. The intensity of blast transformation to PHA did not correlate with the increase of T-lymphocytes in culture. These data suggest that cells of not T-origin may be the limiting factor during mitogenic response of T-lymphocytes to PHA.
从正常供体的肝素化外周血中，通过Ficoll-泛影葡胺梯度分离出富含T细胞的淋巴细胞组分。培养的T淋巴细胞的促有丝分裂反应是未分离单核细胞的3倍。对PHA的母细胞转化强度与培养中T淋巴细胞的增加无关。这些数据表明，非T来源的细胞可能是T淋巴细胞对PHA促有丝分裂反应的限制因素。
