Bohne C, Campa A, Cilento G, Nassi L, Villablanca M
Anal Biochem. 1986 May 15;155(1):1-9. doi: 10.1016/0003-2697(86)90215-0.
Micelle-solubilized chlorophyll efficiently detects electronically excited species generated in enzymatic systems. In most, if not all, systems the chemiexcited species is formed in the triplet state; chlorophyll fluorescence is observed as result of energy transfer. Red emission can also be elicited from chlorophyll in chloroplasts or bound to microsomes.
胶束增溶叶绿素能有效检测酶系统中产生的电子激发态物质。在大多数(即便不是全部)系统中,化学激发态物质以三重态形成;叶绿素荧光是能量转移的结果。叶绿体中或与微粒体结合的叶绿素也能发出红色荧光。