针对牛血型抗原的单克隆抗体。

Monoclonal antibodies to bovine blood group antigens.

作者信息

Tucker E M, Méténier L, Grosclaude J, Clarke S W, Kilgour L

出版信息

Anim Genet. 1986;17(1):3-13. doi: 10.1111/j.1365-2052.1986.tb03183.x.

DOI:10.1111/j.1365-2052.1986.tb03183.x

Abstract

Hybridomas were made by fusing mouse myeloma cells with spleen cells from mice immunized with bovine red cells. Sixteen cloned lines which secreted haemolytic monoclonal antibodies reacting with antigens in the A, B, F, Z and S blood group systems were established; one of the antibodies identified a new factor in the B system. Extensive tests on red cells from 1000 animals indicated that several of the antibodies are suitable for use in routine blood typing; others are of potential use for genetic studies of the bovine blood group systems.

摘要

通过将小鼠骨髓瘤细胞与用牛红细胞免疫的小鼠脾细胞融合制备杂交瘤。建立了16个分泌与A、B、F、Z和S血型系统中的抗原发生反应的溶血型单克隆抗体的克隆系；其中一种抗体在B系统中鉴定出一个新因子。对1000只动物的红细胞进行的广泛测试表明，几种抗体适用于常规血型鉴定；其他抗体对牛血型系统的遗传学研究具有潜在用途。