由重新融合的小鼠/小牛杂交瘤产生的特异性牛单克隆抗体。
Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma.
作者信息
Tucker E M, Dain A R, Clarke S W, Donker R A
出版信息
Hybridoma. 1984 Summer;3(2):171-6. doi: 10.1089/hyb.1984.3.171.
Hybridomas made by fusing NSO (subline NSI Ag 4-1) mouse myeloma cells with lymph node cells from a calf immunized with sheep red blood cells failed to maintain antibody secretion in culture. However, when two of these hybridomas were selected in 8-azaguanine and then re-fused with immunized calf lymph node cells, several lines were obtained that secreted bovine Ig. One cloned line, producing bovine IgG1 (strongly lytic in the presence of rabbit complement and presumed to be an anti-Forssman) was maintained in culture for five months. Cytogenetic studies confirmed that the mouse/calf hybridomas lost bovine chromosomes as they proliferated, but that re-fusion increased the bovine complement from a mean of 5 (2 n) to 11 (2 n) bovine chromosomes per cell. It is proposed that the selected hybridoma lines may be suitable fusion partners for the production of further monoclonal bovine antibodies.
将NSO（亚系NSI Ag 4-1）小鼠骨髓瘤细胞与用绵羊红细胞免疫的小牛淋巴结细胞融合制备的杂交瘤在培养中未能维持抗体分泌。然而，当这些杂交瘤中的两个在8-氮杂鸟嘌呤中进行筛选，然后再与免疫的小牛淋巴结细胞融合时，获得了几个分泌牛免疫球蛋白的细胞系。一个克隆细胞系产生牛IgG1（在兔补体存在下具有强烈的溶细胞作用，推测为抗福斯曼抗体），在培养中维持了五个月。细胞遗传学研究证实，小鼠/小牛杂交瘤在增殖过程中丢失了牛染色体，但再次融合使每个细胞的牛染色体平均数从5条（2n）增加到11条（2n）。有人提出，所选的杂交瘤细胞系可能是生产更多单克隆牛抗体的合适融合伙伴。
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