Hamzah Israa Hussein, Shafi Farha A Ali, Al Sharqi Sahar A H, Brakhas Suaad Almas
1Biology Department, College of Science, Mustansiriyah University, Baghdad, Iraq.
Allergy Specialized Center, Baghdad/Resafa, Iraq.
Clin Mol Allergy. 2019 Mar 5;17:4. doi: 10.1186/s12948-019-0108-0. eCollection 2019.
GST belongs to a super family of phase II detoxification enzyme and it plays an important role in preventing the damage that may occur due to reactive water-soluble compounds generated by the association of reactive intermediates with glutathione.
In the present study, we analyzed the frequencies of GSTP1 polymorphism among the Iraqi population using PCR-RFLP technique. Fifty samples from bronchial asthma patients and fifty samples from control cases were subjected to conventional PCR and Restriction Fragment Length Polymorphism (RFLP) to detect GSTP1 genotype and measured different parameters together such as IgE, eosinophilic count, WBC, and so forth. Some of the cases were made to undergo sequence analysis and enrolled in NCBI GenBank with accession number (MG657249-MG657258). The GSTP1 polymorphism was determined using PCR and the resultant 176-bp fragment was subjected to RFLP and digested with BsamA1 to recognize the A-G transition at nucleotide.
Homozygotes for Ile105 encoding allele resulted in 176-bp fragment found in 62% andVal105 encoding allele had two fragments of 91 and 85 bp in PCR was found in 4% of asthmatic patients. On the other hand, heterozygotes resulted in three fragments of 176, 91 and 85 bp seen in 34% of patients.
To the best of the researcher's knowledge, this is the first-of-its-kind report with regards to the role played by GSTP1 polymorphism in bronchial asthma among the Iraqi patients. Though the study outcomes do not support the large role played by GSTP1 gene polymorphism in the evolution of bronchial asthma disorder, future researchers are suggested to investigate more features for many promising results.
谷胱甘肽 S - 转移酶（GST）属于Ⅱ相解毒酶超家族，在预防因反应性中间体与谷胱甘肽结合产生的反应性水溶性化合物可能造成的损伤方面发挥着重要作用。
在本研究中，我们采用聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）技术分析了伊拉克人群中GSTP1基因多态性的频率。对50份支气管哮喘患者样本和50份对照样本进行常规PCR和限制性片段长度多态性分析（RFLP），以检测GSTP1基因型，并同时测量不同参数，如免疫球蛋白E（IgE）、嗜酸性粒细胞计数、白细胞等。部分病例进行了序列分析，并以登录号（MG657249 - MG657258）录入美国国立生物技术信息中心（NCBI）基因库。使用PCR确定GSTP1基因多态性，将得到的176碱基对片段进行RFLP分析，并用BsamA1酶切以识别核苷酸处的A - G转换。
编码异亮氨酸105的等位基因纯合子产生176碱基对片段，在62%的哮喘患者中被发现；编码缬氨酸105的等位基因在PCR中有91和85碱基对的两个片段，在4%的哮喘患者中被发现。另一方面，杂合子产生176、91和85碱基对的三个片段，在34%的患者中可见。
就研究人员所知，这是关于GSTP1基因多态性在伊拉克患者支气管哮喘中所起作用的首份此类报告。尽管研究结果不支持GSTP1基因多态性在支气管哮喘疾病演变中起很大作用，但建议未来的研究人员进一步研究更多特征以获得更有前景的结果。
