Schwartz J, Suda D, Light G
Biochem Biophys Res Commun. 1986 May 14;136(3):1130-5. doi: 10.1016/0006-291x(86)90451-1.
Beta carotene (250 micrograms/ml) dissolved in mineral oil applied either topically or injected locally (190 ng/ml dissolved in media) into DMBA (7,12-dimethylbenz(a)anthracene)-induced or HCPC-1 cell line-produced oral squamous cell carcinoma of the hamster buccal pouch was observed to result in the regression of these tumors. (p less than or equal to .005) Beta carotene application to tumor bearing pouches was observed to produce a dramatic increase in positively stained macrophages for tumor necrosis factor (TNF-alpha) as compared to macrophages in control pouches. Macrophages from hamsters with regressed tumor were shown to produce a significant increase in cytotoxicity to HCPC-1 tumor cells. Regression of the hamster oral carcinoma was correlated with the increased capacity of macrophages to lyse tumor cells, and related to the induction of tumor necrosis factor which was associated with the administration of the carotenoid, beta carotene.
将溶解于矿物油中的β-胡萝卜素（250微克/毫升）局部外用，或（溶解于培养基中的190纳克/毫升）局部注射到由7,12-二甲基苯并(a)蒽（DMBA）诱导产生或由HCPC-1细胞系生成的仓鼠颊囊口腔鳞状细胞癌中，结果观察到这些肿瘤出现消退。（p小于或等于0.005）与对照颊囊中的巨噬细胞相比，观察到将β-胡萝卜素应用于荷瘤颊囊后，肿瘤坏死因子（TNF-α）阳性染色的巨噬细胞显著增加。结果显示，来自肿瘤消退仓鼠的巨噬细胞对HCPC-1肿瘤细胞的细胞毒性显著增加。仓鼠口腔癌的消退与巨噬细胞裂解肿瘤细胞的能力增强相关，并且与类胡萝卜素β-胡萝卜素给药后诱导产生的肿瘤坏死因子有关。
