Suda D, Schwartz J, Shklar G
Carcinogenesis. 1986 May;7(5):711-5. doi: 10.1093/carcin/7.5.711.
beta-Carotene was found to significantly inhibit the formation of 7,12-dimethylbenz[a]anthracene (DMBA)-induced squamous cell carcinoma of hamster buccal pouch when applied topically on days alternate to the application of 0.25% DMBA in heavy mineral oil thrice weekly for 22 weeks. An initial experiment utilized 40 male young adult Syrian hamsters divided into four equal groups. Group 1 had DMBA applied to left buccal pouches thrice weekly. Group 2 had DMBA applied as in group 1 but also beta-carotene thrice weekly on days alternate to the DMBA application. Group 3 animals were painted with only beta-carotene and group 4 animals were untreated controls. In a second experiment with 80 animals, beta-carotene was found to inhibit oral carcinogenesis in an initiation--promotion hamster buccal pouch system using 0.1% DMBA as initiator and 40% benzoyl peroxide as promoter. beta-Carotene inhibited both initiation and promotion.
当每周三次在重质矿物油中使用0.25%二甲基苯并[a]蒽（DMBA），连续22周，且在交替日局部应用β-胡萝卜素时，发现β-胡萝卜素能显著抑制DMBA诱导的仓鼠颊囊鳞状细胞癌的形成。最初的实验使用了40只雄性成年叙利亚仓鼠，将其分成四个相等的组。第1组每周三次将DMBA应用于左颊囊。第2组与第1组一样应用DMBA，但在与应用DMBA的交替日也每周三次应用β-胡萝卜素。第3组动物仅涂抹β-胡萝卜素，第4组动物为未处理的对照。在一项使用80只动物的第二个实验中，发现在以0.1% DMBA作为引发剂和40%过氧化苯甲酰作为促进剂的启动-促进仓鼠颊囊系统中，β-胡萝卜素可抑制口腔致癌作用。β-胡萝卜素抑制了启动和促进过程。
