Sharifi B G, Bascom C C, Johnson T C
Biochem Biophys Res Commun. 1986 May 14;136(3):976-82. doi: 10.1016/0006-291x(86)90428-6.
The ability of the calcium ionophore A23187 and the sodium ionophore Monensin to antagonize the inhibition of 3T3 cell protein synthesis by a bovine cell surface sialoglycopeptide was measured. A23187, when added before and shortly after the sialoglycopeptide, significantly reduced the biological activity of the inhibitory glycopeptide. In contrast, Monensin had little, if any, influence on protein synthesis inhibition by the sialoglycopeptide. The ability of A23187 to circumvent the inhibitory action of the bovine glycopeptide was shown to be independent of the time the ionophore was incubated with the cells and the binding of the sialoglycopeptide to the 3T3 target cells. Neither the total amount of sialoglycopeptide bound to the cells, nor its affinity to the cell surface receptor, was influenced by the presence of A23187.
测定了钙离子载体A23187和钠离子载体莫能菌素拮抗牛细胞表面唾液酸糖肽对3T3细胞蛋白质合成抑制作用的能力。在唾液酸糖肽加入之前及之后不久加入A23187，可显著降低抑制性糖肽的生物活性。相比之下，莫能菌素对唾液酸糖肽抑制蛋白质合成的作用几乎没有影响（如果有影响的话也很小）。结果表明，A23187规避牛糖肽抑制作用的能力与离子载体与细胞孵育的时间以及唾液酸糖肽与3T3靶细胞的结合无关。A23187的存在既不影响与细胞结合的唾液酸糖肽的总量，也不影响其对细胞表面受体的亲和力。
