Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Pest Manag Sci. 2019 Nov;75(11):2969-2974. doi: 10.1002/ps.5409. Epub 2019 Apr 10.
Permethrin (a pyrethroid) has been recommended for the management of a number of insect pests, including Musca domestica. Recently, resistance to permethrin has been reported from the Punjab province, Pakistan, which necessitates the need to manage the resistance problem. For this reason, a field strain of M. domestica was selected with permethrin for ten consecutive generations (G1-G10) to determine the risk assessment, the rapidity with which permethrin resistance can develop, and the presence or absence of the cross-resistance phenomenon.
The results revealed that permethrin resistance increased very quickly in response to selection experiments. The resistance ratio (RR) value increased by 16.04- to 605.92-fold and the LD value increased by 100.22 to 3787.08 ng fly after selection experiments. Moreover, the increase in LD value remained stable when the selected strain (Perm-SEL) was cultured in the absence of permethrin for the next five generations (G11-G15). The results revealed the possibility of minor cross-resistance in the Perm-SEL strain with β-cyfluthrin and deltamethrin, and lack of cross-resistance with imidacloprid and spinosad. The realized heritability (h ) value for the Perm-SEL strain was 0.23. Assuming a mean slope value of 1.27 and an h value of 0.23, then ten to four generations would be estimated for a 10-fold increase in the LD values at 50-90% selection intensity, respectively.
The results of present study revealed high risk for permethrin resistance development under continuous selection pressure. Permethrin resistance developed very quickly under laboratory conditions, which might reflect the likelihood of resistance evolution in field conditions if permethrin selection pressure holds for a long time. © 2019 Society of Chemical Industry.
拟除虫菊酯（一种拟除虫菊酯）已被推荐用于防治包括家蝇在内的多种害虫。最近，巴基斯坦旁遮普省报告了对拟除虫菊酯的抗性，这就需要管理抗性问题。为此，选择了家蝇的田间种群，用拟除虫菊酯连续选择了十代（G1-G10），以确定风险评估、拟除虫菊酯抗性快速发展的速度以及是否存在交叉抗性现象。
结果表明，拟除虫菊酯抗性在选择实验中迅速增加。抗性比（RR）值增加了 16.04-605.92 倍，LD 值增加了 100.22-3787.08ng 苍蝇，选择实验后。此外，当选择的菌株（Perm-SEL）在没有拟除虫菊酯的情况下连续培养五代（G11-G15）时，LD 值的增加仍然稳定。结果表明，Perm-SEL 菌株与β-氯氟氰菊酯和溴氰菊酯存在轻微的交叉抗性，与吡虫啉和多杀菌素无交叉抗性。Perm-SEL 菌株的现实遗传力（h）值为 0.23。假设平均斜率值为 1.27，h 值为 0.23，那么在 50-90%选择强度下，LD 值增加 10 倍需要 10-4 代。
本研究结果表明，在持续选择压力下，拟除虫菊酯抗性发展的风险很高。在实验室条件下，拟除虫菊酯抗性迅速发展，这可能反映出如果拟除虫菊酯选择压力持续很长时间，野外条件下抗性进化的可能性。 © 2019 化学工业协会。
