Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education , Jiangnan University , Wuxi 214122 , P. R. China.
School of Biotechnology , Jiangnan University , Wuxi 214122 , P. R. China.
J Agric Food Chem. 2019 Apr 10;67(14):3900-3908. doi: 10.1021/acs.jafc.9b00227. Epub 2019 Mar 29.
In this study, we investigated the effects of the different critical genes in the three modules on tyrosol production in Escherichia coli. Coexpression of the yahK and ARO10 genes increased the yield of tyrosol by 10% compared to that of the control. Tyrosol production by E. coli BFPT1 and E. coli BFPA1 was higher by 15.0% and 17.8% than that by the control, respectively, via coordinated expression of key genes from modules 2 and 3. The tyrosol yield of E. coli BFPE2 was 58.3% higher than that of the control (reaching 5.72 mM) when the expression levels of the key genes aroA and tyrA* from module 2 were balanced. The tyrosol yield of E. coli BFPG1 was increased by 52.6% (reaching 5.8 mM) compared to the control via coexpression of modules 1, 2, and 3. This work suggested that microbial production of tyrosol in E. coli has potential for industrial applications.
在本研究中，我们研究了三个模块中不同关键基因对大肠杆菌中天冬氨酸产量的影响。与对照组相比，yahK 和 ARO10 基因的共表达使天冬氨酸产量提高了 10%。通过协调表达模块 2 和 3 的关键基因，E. coli BFPT1 和 E. coli BFPA1 的天冬氨酸产量分别比对照组提高了 15.0%和 17.8%。当模块 2 中的关键基因 aroA 和 tyrA* 的表达水平平衡时，E. coli BFPE2 的天冬氨酸产量比对照组提高了 58.3%（达到 5.72mM）。通过共表达模块 1、2 和 3，E. coli BFPG1 的天冬氨酸产量比对照组提高了 52.6%（达到 5.8mM）。这项工作表明，大肠杆菌中天冬氨酸的微生物生产具有工业应用的潜力。
