a Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology , Sun Yat-sen University , Guangzhou , China.
b Xianning Central Hospital , The First Affiliated Hospital Of Hubei University Of Science And Technology , Xianning , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):822-832. doi: 10.1080/21691401.2019.1576706.
Graphene and its derivatives, graphene oxide (GO) and graphene oxide quantum dots (GOQDs), have recently attracted much attention as bioactive factors in differentiating stem cells towards osteoblastic lineage. The stem cells from human exfoliated deciduous teeth (SHEDs) possess the properties of self-renewal, extensive proliferation, and multiple differentiation potential, and have gradually become one of the most promising mesenchymal stem cells (MSCs) in bone tissue engineering. The purpose of this study was to explore the effects of GO and GOQDs on the osteogenic differentiation of SHEDs. In this study, GO and GOQDs facilitated SHED proliferation up to 7 days in vitro at the concentration of 1 μg/ml. Because of their excellent fluorescent properties, GOQD uptake by SHEDs was confirmed and distributed in the SHED cytoplasm. Calcium nodules formation, alkaline phosphatase (ALP) activity, and RNA and protein expression increased significantly in SHEDs treated with osteogenic induction medium containing GOQDs but decreased with osteogenic induction medium containing GO. Interestingly, the Wnt/β-catenin signaling pathway appeared to be involved in osteogenic differentiation of SHEDs induced with GOQDs. In summary, GO and GOQDs at the concentration of 1 μg/ml promoted SHED proliferation. GOQDs induced the osteogenic differentiation of SHEDs, whilst GO slightly inhibited it.
石墨烯及其衍生物，氧化石墨烯（GO）和氧化石墨烯量子点（GOQDs），作为分化干细胞为成骨细胞系的生物活性因子，近来受到广泛关注。人脱落乳牙干细胞（SHEDs）具有自我更新、广泛增殖和多向分化潜能的特性，逐渐成为骨组织工程中最有前途的间充质干细胞（MSCs）之一。本研究旨在探讨 GO 和 GOQDs 对 SHEDs 成骨分化的影响。在这项研究中，GO 和 GOQDs 在 1μg/ml 的浓度下促进 SHED 体外增殖长达 7 天。由于其出色的荧光特性，证实了 GOQD 被 SHEDs 摄取，并分布在 SHED 细胞质中。用含有 GOQDs 的成骨诱导培养基处理的 SHEDs 中钙结节形成、碱性磷酸酶（ALP）活性以及 RNA 和蛋白表达显著增加，而用含有 GO 的成骨诱导培养基处理的 SHEDs 则减少。有趣的是，Wnt/β-catenin 信号通路似乎参与了 GOQDs 诱导的 SHEDs 成骨分化。总之，浓度为 1μg/ml 的 GO 和 GOQDs 促进了 SHED 的增殖。GOQDs 诱导 SHEDs 成骨分化，而 GO 则略有抑制作用。
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