Xu Duoling, Wang Chao, Wu Jie, Fu Yuanxiang, Li Shujun, Hou Wentao, Lin Ling, Li Pei, Yu Dongsheng, Zhao Wei
Hospital of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, P. R. China.
Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510050, P. R. China.
ACS Omega. 2022 Apr 18;7(16):13546-13556. doi: 10.1021/acsomega.1c06892. eCollection 2022 Apr 26.
Graphene oxide quantum dots (GOQDs) are considered to be a new method for regulating the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are few reports on such regulation with different concentrations of GOQDs, and the molecular mechanism has not been fully elucidated. The purposes of this study were, first, to explore the effects of GOQDs on the proliferation and differentiation of BMSCs and , and, second, to provide a theoretical basis for the repair of bone defects. Live/Dead staining, EdU staining, immunofluorescence staining, alkaline phosphatase (ALP), western blotting, and qT-PCR were used for detecting the proliferation and differentiation of BMSCs after coculture with GOQDs of different concentrations. Hematoxylin and eosin (HE) staining and Van Gieson (VG) staining were used to detect new bone regeneration . The results showed that low-concentration GOQDs (0.1 and 1 μg/mL) promoted the proliferation and differentiation of BMSCs. Compared with the 1 μg/mL GOQD group, the 0.1 μg/mL GOQD group had better ability to promote the proliferation and differentiation of BMSCs. HE and VG staining results showed the greatest proportion of new bone area on sandblasted, large-grit, and acid-etched (SLA)/GOQD scaffolds. Furthermore, the ratio of active β-catenin and the phosphorylation level of GSK-3β (p-GSK-3β) increased after BMSCs treatment with 0.1 μg/mL GOQDs. Low concentrations of GOQDs improved the osteogenic differentiation ability of BMSCs by activating the Wnt/β-catenin signaling pathway.
氧化石墨烯量子点（GOQDs）被认为是调节骨髓间充质干细胞（BMSCs）增殖和分化的一种新方法。然而，关于不同浓度GOQDs的这种调节作用的报道较少，其分子机制也尚未完全阐明。本研究的目的，一是探究GOQDs对BMSCs增殖和分化的影响，二是为骨缺损修复提供理论依据。采用活/死染色、EdU染色、免疫荧光染色、碱性磷酸酶（ALP）、蛋白质免疫印迹法和定量逆转录聚合酶链反应（qT-PCR）检测不同浓度GOQDs与BMSCs共培养后BMSCs的增殖和分化情况。采用苏木精-伊红（HE）染色和范吉森（VG）染色检测新骨再生情况。结果显示，低浓度GOQDs（0.1和1μg/mL）促进了BMSCs的增殖和分化。与1μg/mL GOQD组相比，0.1μg/mL GOQD组促进BMSCs增殖和分化的能力更强。HE和VG染色结果显示，喷砂、大颗粒、酸蚀（SLA）/GOQD支架上的新骨面积比例最大。此外，用0.1μg/mL GOQDs处理BMSCs后，活性β-连环蛋白的比例和糖原合成酶激酶-3β（p-GSK-3β）的磷酸化水平升高。低浓度GOQDs通过激活Wnt/β-连环蛋白信号通路提高了BMSCs的成骨分化能力。
